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. 1982 Nov;38(2):651–667. doi: 10.1128/iai.38.2.651-667.1982

Bacteriology of experimental gingivitis in young adult humans.

W E Moore, L V Holdeman, R M Smibert, I J Good, J A Burmeister, K G Palcanis, R R Ranney
PMCID: PMC347789  PMID: 7141708

Abstract

From replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. Of these bacteria, Actinomyces naeslundii (serotype III and phenotypically similar strains that were unreactive with available antisera), Actinomyces odontolyticus (serotype I and phenotypically similar strains that were unreactive with available antisera), Fusobacterium nucleatum, Lactobacillus species D-2, Streptococcus anginosus, Veillonella parvula, and Treponema species A appeared to be the most likely etiological agents of gingivitis. Statistical interpretations indicated that the greatest source of microbiological variation of the total flora observed was person-to-person differences in the floras. The next greatest source of variation was the inflammatory status of the sample sites. Person-to-person differences were smallest at experimental day 4. The floras became more diverse with time and as gingivitis developed and progressed. Analyses indicated that sequential colonization by certain species was repeatable and therefore probably predictable. Variation was relatively small between replicate trials, between two sites on the same teeth sampled on the same day, and between the same sites sampled at the same relative time in a replicate trial.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bick P. H., Carpenter A. B., Holdeman L. V., Miller G. A., Ranney R. R., Palcanis K. G., Tew J. G. Polyclonal B-cell activation induced by extracts of Gram-negative bacteria isolated from periodontally diseased sites. Infect Immun. 1981 Oct;34(1):43–49. doi: 10.1128/iai.34.1.43-49.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bladen H., Hageage G., Pollock F., Harr R. Plaque formation in vitro on wires by gram-negative oral microorganisms (Veillonella). Arch Oral Biol. 1970 Feb;15(2):127–133. doi: 10.1016/0003-9969(70)90048-8. [DOI] [PubMed] [Google Scholar]
  3. Gibbons R. J., Nygaard M. Interbacterial aggregation of plaque bacteria. Arch Oral Biol. 1970 Dec;15(12):1397–1400. doi: 10.1016/0003-9969(70)90031-2. [DOI] [PubMed] [Google Scholar]
  4. Holdeman L. V., Good I. J., Moore W. E. Human fecal flora: variation in bacterial composition within individuals and a possible effect of emotional stress. Appl Environ Microbiol. 1976 Mar;31(3):359–375. doi: 10.1128/aem.31.3.359-375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holm-Pedersen P., Agerbaek N., Theilade E. Experimental gingivitis in young and elderly individuals. J Clin Periodontol. 1975 Feb;2(1):14–24. doi: 10.1111/j.1600-051x.1975.tb01722.x. [DOI] [PubMed] [Google Scholar]
  6. Kelstrup J., Funder-Nielsen T. D. Aggregation of oral streptococci with Fusobacterium and Actinomyces. J Biol Buccale. 1974 Dec;2(4):347–362. [PubMed] [Google Scholar]
  7. LOE H., THEILADE E., JENSEN S. B. EXPERIMENTAL GINGIVITIS IN MAN. J Periodontol. 1965 May-Jun;36:177–187. doi: 10.1902/jop.1965.36.3.177. [DOI] [PubMed] [Google Scholar]
  8. Loesche W. J., Syed S. A. Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score. Infect Immun. 1978 Sep;21(3):830–839. doi: 10.1128/iai.21.3.830-839.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moore W. E., Hash D. E., Holdeman L. V., Cato E. P. Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl Environ Microbiol. 1980 Apr;39(4):900–907. doi: 10.1128/aem.39.4.900-907.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moore W. E., Holdeman L. V. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol. 1974 May;27(5):961–979. doi: 10.1128/am.27.5.961-979.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Payne W. A., Page R. C., Ogilvie A. L., Hall W. B. Histopathologic features of the initial and early stages of experimental gingivitis in man. J Periodontal Res. 1975 May;10(2):51–64. doi: 10.1111/j.1600-0765.1975.tb00008.x. [DOI] [PubMed] [Google Scholar]
  12. Peros W. J., Gibbons R. J. Influence of sublethal antibiotic concentrations on bacterial adherence to saliva-treated hydroxyapatite. Infect Immun. 1982 Jan;35(1):326–334. doi: 10.1128/iai.35.1.326-334.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SILNESS J., LOE H. PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. Acta Odontol Scand. 1964 Feb;22:121–135. doi: 10.3109/00016356408993968. [DOI] [PubMed] [Google Scholar]
  14. Singer R. E., Buckner B. A. Butyrate and propionate: important components of toxic dental plaque extracts. Infect Immun. 1981 May;32(2):458–463. doi: 10.1128/iai.32.2.458-463.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Socransky S. S., Holt S. C., Leadbetter E. R., Tanner A. C., Savitt E., Hammond B. F. Capnocytophaga: new genus of gram-negative gliding bacteria. III. Physiological characterization. Arch Microbiol. 1979 Jul;122(1):29–33. doi: 10.1007/BF00408042. [DOI] [PubMed] [Google Scholar]
  16. Syed S. A., Loesche W. J. Bacteriology of human experimental gingivitis: effect of plaque age. Infect Immun. 1978 Sep;21(3):821–829. doi: 10.1128/iai.21.3.821-829.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Theilade E., Wright W. H., Jensen S. B., Löe H. Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation. J Periodontal Res. 1966;1:1–13. doi: 10.1111/j.1600-0765.1966.tb01842.x. [DOI] [PubMed] [Google Scholar]
  18. Williams B. L., Hollis D., Holdeman L. V. Synonymy of strains of Center for Disease Control group DF-1 with species of Capnocytophaga. J Clin Microbiol. 1979 Oct;10(4):550–556. doi: 10.1128/jcm.10.4.550-556.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zachrisson B. U. A histological study of experimental gingivitis in man. J Periodontal Res. 1968;3(4):293–302. doi: 10.1111/j.1600-0765.1968.tb01935.x. [DOI] [PubMed] [Google Scholar]

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