Abstract
Hybridomas raised against two Papua New Guinea (PNG) isolates of Plasmodium falciparum secreted monoclonal antibodies which bound to schizonts of all seven PNG isolates tested but not to schizonts of four non-PNG isolates from Thailand, Nigeria, Ghana, and The Netherlands. Some of the monoclonal antibodies were tested for their ability to inhibit the growth of one PNG isolate, one Thai isolate, and one Nigerian isolate in vitro. Only the growth of the PNG isolate was inhibited, thus demonstrating functional antigenic differences among isolates of P. falciparum.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
- Chen P., Lamont G., Elliott T., Kidson C., Brown G., Mitchell G., Stace J., Alpers M. Plasmodium falciparum strains from Papua New Guinea: culture characteristics and drug sensitivity. Southeast Asian J Trop Med Public Health. 1980 Dec;11(4):435–440. [PubMed] [Google Scholar]
- Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
- Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
- Lambros C., Vanderberg J. P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979 Jun;65(3):418–420. [PubMed] [Google Scholar]
- Langreth S. G., Reese R. T., Motyl M. R., Trager W. Plasmodium falciparum: loss of knobs on the infected erythrocyte surface after long-term cultivation. Exp Parasitol. 1979 Oct;48(2):213–219. doi: 10.1016/0014-4894(79)90101-2. [DOI] [PubMed] [Google Scholar]
- Myler P., Saul A., Mangan T., Kidson C. An automated assay of merozoite invasion of erythrocytes using highly synchronized Plasmodium falciparum cultures. Aust J Exp Biol Med Sci. 1982 Feb;60(Pt 1):83–89. doi: 10.1038/icb.1982.6. [DOI] [PubMed] [Google Scholar]
- Perrin L. H., Dayal R. Immunity to asexual erythrocytic stages of Plasmodium falciparum: role of defined antigens in the humoral response. Immunol Rev. 1982;61:245–269. doi: 10.1111/j.1600-065x.1982.tb00379.x. [DOI] [PubMed] [Google Scholar]
- Richards W. H., Maples B. K. Studies on Plasmodium falciparum in continuous cultivation. I. The effect of chloroquine and pyrimethamine on parasite growth and viability. Ann Trop Med Parasitol. 1979 Apr;73(2):99–108. [PubMed] [Google Scholar]
- Sadun E. H., Hickman R. L., Wellde B. T., Moon A. P., Udeozo I. O. Active and passive immunization of chimpanzees infected with West African and Southeast Asian strains of Plasmodium falciparum. Mil Med. 1966 Sep;131(9 Suppl):1250–1262. [PubMed] [Google Scholar]
- Sanderson A., Walliker D., Molez J. F. Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans R Soc Trop Med Hyg. 1981;75(2):263–267. doi: 10.1016/0035-9203(81)90331-x. [DOI] [PubMed] [Google Scholar]
- Thaithong S., Beale G. H. Resistance of ten Thai isolates of Plasmodium falciparum to chloroquine and pyrimethamine by in vitro tests. Trans R Soc Trop Med Hyg. 1981;75(2):271–273. doi: 10.1016/0035-9203(81)90333-3. [DOI] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
- Wilson R. J., Phillips R. S. Method to test inhibitory antibodies in human sera to wild populations of Plasmodium falciparum. Nature. 1976 Sep 9;263(5573):132–134. doi: 10.1038/263132a0. [DOI] [PubMed] [Google Scholar]