Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Dec;38(3):907–913. doi: 10.1128/iai.38.3.907-913.1982

Regulatory Interactions Between Macrophages and T-Cell Subsets in Listeria monocytogenes-Specific T-Cell Activation

Stefan H E Kaufmann 1,†, Markus M Simon 2, Helmut Hahn 1
PMCID: PMC347835  PMID: 6818150

Abstract

Peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice in the presence of the homologous antigen (heat-killed L. monocytogenes) and normal macrophages showed L. monocytogenes-specific proliferative responses. Proliferation was inhibited by macrophages from L. monocytogenes- or Corynebacterium parvum-pretreated mice as well as by exogenous prostaglandin E2. Macrophage-dependent inhibition of T-cell proliferation—at least in part—could be reversed by addition of indomethacin. When selected L. monocytogenes-immune Lyt T-cell subsets were cultured in the presence of inhibitory macrophages, pretreatment with anti-Lyt 1 antiserum plus complement completely abrogated proliferation and pretreatment with anti-Lyt 2 and anti-Lyt 3 antisera plus complement markedly reduced proliferation. However, a mixture (1:1) of the two preselected Lyt T-cell subsets resulted in complete reconstitution of proliferative responses. In contrast, when L. monocytogenes-immune peritoneal exudate T lymphocytes were treated with anti-Lyt antisera plus complement after culture, only treatment with anti-Lyt 1 antiserum plus complement affected proliferation, suggesting regulatory interactions between Lyt 1+23− and Lyt 1−23+ T cells during in vitro culture which result in proliferation within the Lyt 1+23− T-cell subset. After rigorous depletion of residual macrophages and in the presence of indomethacin, pretreatment with anti-Lyt 1 antiserum plus complement, but not with anti-Lyt 2 and 3 antisera plus complement, eliminated proliferation. The data presented indicate that interactions between macrophages and Lyt T-cell subsets regulate L. monocytogenes-specific T-cell activation.

Full text

PDF
907

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Gershon R. K. Immunological circuits: cellular composition. Fed Proc. 1979 Jun;38(7):2058–2064. [PubMed] [Google Scholar]
  2. Currie G. A. Activated macrophages kill tumour cells by releasing arginase. Nature. 1978 Jun 29;273(5665):758–759. doi: 10.1038/273758a0. [DOI] [PubMed] [Google Scholar]
  3. Farr A. G., Kiely J. M., Unanue E. R. Macrophage-T cell interactions involving Listeria monocytogenes--role of the H-2 gene complex. J Immunol. 1979 Jun;122(6):2395–2404. [PubMed] [Google Scholar]
  4. Goronzy J., Schaefer U., Eichmann K., Simon M. M. Quantitative studies on T cell diversity. II. Determination of the frequencies and Lyt phenotypes of two types of precursor cells for alloreactive cytotoxic T cells in polyclonally and specifically activated splenic T cells. J Exp Med. 1981 Apr 1;153(4):857–870. doi: 10.1084/jem.153.4.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hahn H., Kaufmann S. H., Falkenberg F., Chahinin M., Horn W. Peritoneal exudate T lymphocytes with specificity to sheep red blood cells. II. Inflammatory helper T cells and effector T cells in mice with delayed-type hypersensitivity and in suppressed mice. Immunology. 1979 Sep;38(1):51–55. [PMC free article] [PubMed] [Google Scholar]
  6. Hahn H., Kaufmann S. H. The role of cell-mediated immunity in bacterial infections. Rev Infect Dis. 1981 Nov-Dec;3(6):1221–1250. doi: 10.1093/clinids/3.6.1221. [DOI] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. Kaufmann S. H., Hahn H. Biological functions of t cell lines with specificity for the intracellular bacterium Listeria monocytogenes in vitro and in vivo. J Exp Med. 1982 Jun 1;155(6):1754–1765. doi: 10.1084/jem.155.6.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaufmann S. H., Hahn H., Simon M. M., Röllinghoff M., Wagner H. Interleukin 2 induction in Lyt 1+ 23- T cells from Listeria monocytogenes-immune mice. Infect Immun. 1982 Sep;37(3):1292–1294. doi: 10.1128/iai.37.3.1292-1294.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaufmann S. H., Simon M. M., Hahn H. Specific Lyt 123 cells are involved in protection against Listeria monocytogenes and in delayed-type hypersensitivity to listerial antigens. J Exp Med. 1979 Oct 1;150(4):1033–1038. doi: 10.1084/jem.150.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kung J. T., Brooks S. B., Jakway J. P., Leonard L. L., Talmage D. W. Suppression of in vitro cytotoxic response by macrophages due to induced arginase. J Exp Med. 1977 Sep 1;146(3):665–672. doi: 10.1084/jem.146.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olds G. R., Ellner J. J., Kearse L. A., Jr, Kazura J. W., Mahmoud A. A. Role of arginase in killing of schistosomula of Schistosoma mansoni. J Exp Med. 1980 Jun 1;151(6):1557–1562. doi: 10.1084/jem.151.6.1557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Simon M. M., Eichmann K. T cell subsets participating in the generation of cytotoxic T cells. Springer Semin Immunopathol. 1980 May;3(1):39–62. doi: 10.1007/BF00199925. [DOI] [PubMed] [Google Scholar]
  15. Zinkernagel R. M., Althage A., Adler B., Blanden R. V., Davidson W. F., Kees U., Dunlop M. B., Shreffler D. C. H-2 restriction of cell-mediated immunity to an intracellular bacterium: effector T cells are specific for Listeria antigen in association with H-21 region-coded self-markers. J Exp Med. 1977 May 1;145(5):1353–1367. doi: 10.1084/jem.145.5.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES