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. 1982 Dec;38(3):975–980. doi: 10.1128/iai.38.3.975-980.1982

Effect of staphylococcal alpha-toxin on phagocytosis of staphylococci by human polymorphonuclear leukocytes.

C G Gemmell, P K Peterson, D J Schmeling, P G Quie
PMCID: PMC347845  PMID: 7152682

Abstract

Evidence is presented which shows that alpha-toxin elaborated by Staphylococcus aureus can affect certain of the biological functions of human polymorphonuclear leukocytes (PMN). The pretreatment of PMNs with low doses (less than 10 hemolytic units) of purified toxin enhanced their ability to phagocytose and to kill serum-opsonized staphylococci. At higher doses (greater than or equal to 10 hemolytic units), a certain amount of cell damage was caused (detectable by trypan blue uptake), which resulted in a reduction of their phagocytic capacity. These effects were not seen either with heat-inactivated (60 degrees C, 30 min) or with antibody-neutralized toxin. It is thought that the toxin can bind onto the membrane of the PMN, either exposing additional receptor sites for opsonized bacteria or speeding up the transport of staphylococci across the membrane. Once ingested, the bacteria were more readily killed by the PMNs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHEIMER A. W., SCHWARTZ L. L. Isolation and composition of staphylococcal alpha toxin. J Gen Microbiol. 1963 Mar;30:455–468. doi: 10.1099/00221287-30-3-455. [DOI] [PubMed] [Google Scholar]
  2. Bernheimer A. W., Schwartz L. L. Lysosomal disruption by bacterial toxins. J Bacteriol. 1964 May;87(5):1100–1104. doi: 10.1128/jb.87.5.1100-1104.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GLADSTONE G. P., VAN HEYNINGEN W. E. Staphylococcal leucocidins. Br J Exp Pathol. 1957 Apr;38(2):123–137. [PMC free article] [PubMed] [Google Scholar]
  4. Gemmell C. G., Peterson P. K., Townsend K., Quie P. G., Kim Y. Biological effects of the interaction of staphylococcal alpha-toxin with human serum. Infect Immun. 1982 Dec;38(3):981–985. doi: 10.1128/iai.38.3.981-985.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gladstone G. P., Yoshida A. The cytopathic action of purified staphylococcal delta-hemolysin. Br J Exp Pathol. 1967 Feb;48(1):11–19. [PMC free article] [PubMed] [Google Scholar]
  6. HIRSCH J. G., BERNHEIMER A. W., WEISSMANN G. MOTION PICTURE STUDY OF THE TOXIC ACTION OF STREPTOLYSINS ON LEUCOCYTES. J Exp Med. 1963 Aug 1;118:223–228. doi: 10.1084/jem.118.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JACKSON A. W., LITTLE R. M. Leucocidal effect of staphylococcal delta-lysin. Can J Microbiol. 1957 Feb;3(1):101–102. doi: 10.1139/m57-012. [DOI] [PubMed] [Google Scholar]
  8. LOMINSKI I., ARBUTHNOTT J. P. Some characteristics of Staphylococcus alpha haemolysin. J Pathol Bacteriol. 1962 Apr;83:515–520. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Maheswaran S. K., Frommes S. P., Lindorfer R. K. Ultrastructural changes in neutrophils treated with staphylococcal alpha toxin. Can J Microbiol. 1969 Jan;15(1):128–129. doi: 10.1139/m69-020. [DOI] [PubMed] [Google Scholar]
  11. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  12. Nonoyama S., Kojo H., Mine Y., Nishida M., Goto S., Kuwahara S. Inhibitory effect of Pseudomonas aeruginosa on the phagacytic and killing activities of rabbit polymorphonuclear leukocytes: purification and characterization of an inhibitor of polymorphonuclear leukocyte function. Infect Immun. 1979 May;24(2):394–398. doi: 10.1128/iai.24.2.394-398.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nonoyama S., Kojo H., Mine Y., Nishida M., Goto S., Kuwahara S. Inhibitory effect of Pseudomonas aeruginosa on the phagocytic and killing activity of rabbit polymorphonuclear leukocytes: mechanisms of action of a polymorphonuclear leukocyte inhibitor. Infect Immun. 1979 May;24(2):399–403. doi: 10.1128/iai.24.2.399-403.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ofek I., Bergner-Rabinowitz S., Ginsburg I. Oxygen-stable hemolysins of group A streptococci. 8. Leukotoxic and antiphagocytic effects of streptolysins S and O. Infect Immun. 1972 Oct;6(4):459–464. doi: 10.1128/iai.6.4.459-464.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ofek I., Bergner-Rabinowitz S., Ginsburg I. Oxygen-stable hemolysins of group A streptococci. VII. The relation of the leukotoxic factor to streptolysin S. J Infect Dis. 1970 Dec;122(6):517–522. doi: 10.1093/infdis/122.6.517. [DOI] [PubMed] [Google Scholar]
  16. Peterson P. K., Verhoef J., Schmeling D., Quie P. G. Kinetics of phagocytosis and bacterial killing by human polymorphonuclear leukocytes and monocytes. J Infect Dis. 1977 Oct;136(4):502–509. doi: 10.1093/infdis/136.4.502. [DOI] [PubMed] [Google Scholar]
  17. Proctor R. A. Endotoxin in vitro interactions with human neutrophils: depression of chemiluminescence, oxygen consumption, superoxide production, and killing. Infect Immun. 1979 Sep;25(3):912–921. doi: 10.1128/iai.25.3.912-921.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schmeling D. J., Gemmell C. G., Craddock P. R., Quie P. G., Peterson P. K. Effect of staphylococcal alpha-toxin on neutrophil migration and adhesiveness. Inflammation. 1981 Dec;5(4):313–322. doi: 10.1007/BF00911095. [DOI] [PubMed] [Google Scholar]
  19. Verhoef J., Peterson P. K., Quie P. G. Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. J Immunol Methods. 1977;14(3-4):303–311. doi: 10.1016/0022-1759(77)90141-7. [DOI] [PubMed] [Google Scholar]
  20. Woodin A. M., Wieneke A. A. The interaction of eucocidin with the cell membrane of the polymorponuclearleucocyte. Biochem J. 1966 May;99(2):479–492. doi: 10.1042/bj0990479. [DOI] [PMC free article] [PubMed] [Google Scholar]

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