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On and off states of tetO7-TATACYC1 vs. Pnmt4 and Pnmt8. A comparison of top1 expression driven from Pnmt4, Pnmt8 and tetO7-TATACYC1 under repressed and induced conditions, as determined by analyzing FLAG3-Top1 protein levels by western blotting. Pnmt4/8 were induced by growing cells in medium lacking thiamine for at least 24 h. tetO7-TATACYC1 was de-repressed by propagating cells in 2.5 μg mL−1 ahTet for at least 6 h.
