Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jan;39(1):155–158. doi: 10.1128/iai.39.1.155-158.1983

Purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera.

S Matsuno, S Inouye
PMCID: PMC347918  PMID: 6295955

Abstract

An outer capsid glycoprotein, VP 7, was purified from a bovine rotavirus, neonatal calf diarrhea virus, by isoelectric focusing in glycerol gradients after disruption of the outer capsid. Its isoelectric point was found to be about 4.5. Guinea pigs were immunized with this VP 7 preparation. The antisera possessed both neutralizing and hemagglutination-inhibiting activities as well as complement-fixing activity, suggesting that VP 7 is a protein involved in hemagglutination and initiation of infection. When these antisera were reacted with a simian rotavirus, their antibody titers were low by hemagglutination-inhibiting and complement-fixing assays, but one serum neutralized the simian rotavirus as efficiently as it did homologous neonatal calf diarrhea virus.

Full text

PDF
155

Images in this article

156Image
on p.156
156Image
on p.156

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infect Immun. 1981 Dec;34(3):641–647. doi: 10.1128/iai.34.3.641-647.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beards G. M., Pilfold J. N., Thouless M. E., Flewett T. H. Rotavirus serotypes by serum neutralisation. J Med Virol. 1980;5(3):231–237. doi: 10.1002/jmv.1890050307. [DOI] [PubMed] [Google Scholar]
  3. Bridger J. C. Location of type-specific antigens in calf rotaviruses. J Clin Microbiol. 1978 Dec;8(6):625–628. doi: 10.1128/jcm.8.6.625-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen J. Ribonucleic acid polymerase activity associated with purified calf rotavirus. J Gen Virol. 1977 Sep;36(3):395–402. doi: 10.1099/0022-1317-36-3-395. [DOI] [PubMed] [Google Scholar]
  5. Fauvel M., Spence L., Babiuk L. A., Petro R., Bloch S. Hemagglutination and hemagglutination-inhibition studies with a strain of Nebraska calf diarrhea virus (bovine rotavirus). Intervirology. 1978;9(2):95–105. doi: 10.1159/000148927. [DOI] [PubMed] [Google Scholar]
  6. Hayes E. C., Lee P. W., Miller S. E., Joklik W. K. The interaction of a series of hybridoma IgGs with reovirus particles. Demonstration that the core protein lambda 2 is exposed on the particle surface. Virology. 1981 Jan 15;108(1):147–155. doi: 10.1016/0042-6822(81)90534-1. [DOI] [PubMed] [Google Scholar]
  7. Korant B. D., Lonberg-Holm K. Zonal electrophoresis and isoelectric focusing of proteins and virus particles in density gradients of small volume. Anal Biochem. 1974 May;59(1):75–82. doi: 10.1016/0003-2697(74)90011-6. [DOI] [PubMed] [Google Scholar]
  8. Matsuno S., Hasegawa A., Kalica A. R., Kono R. Isolation of a recombinant between simian and bovine rotaviruses. J Gen Virol. 1980 May;48(1):253–256. doi: 10.1099/0022-1317-48-1-253. [DOI] [PubMed] [Google Scholar]
  9. Matsuno S., Inouye S., Kono R. Antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests. Infect Immun. 1977 Sep;17(3):661–662. doi: 10.1128/iai.17.3.661-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matsuno S., Inouye S., Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. doi: 10.1128/jcm.5.1.1-4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Matsuno S., Mukoyama A. Polypeptides of bovine rotavirus. J Gen Virol. 1979 May;43(2):309–316. doi: 10.1099/0022-1317-43-2-309. [DOI] [PubMed] [Google Scholar]
  12. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  13. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES