Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jan;39(1):220–224. doi: 10.1128/iai.39.1.220-224.1983

Transformed and normal cell surface glycoproteins that induce interferon production by nonsensitized lymphocytes.

E M Smith, T K Hughes, J E Blalock
PMCID: PMC347929  PMID: 6185432

Abstract

Nonsensitized human and mouse lymphocytes produce interferon (IFN)-alpha and IFN-alpha/beta, respectively, in response to most transformed cells, as well as normal xenogeneic cells. The treatment of transformed human (WISH) and mouse (L) or normal mouse embryo (ME) cells with trypsin, pepsin, or neuraminidase resulted in a loss of the cell's ability to induce IFN-alpha or alpha/beta. These findings suggested that a cell surface glycoprotein is responsible for the induction of IFN-alpha and alpha/beta. The sonication of WISH, L, and ME cells released glycoproteins which retained IFN-inducing activity in the soluble form. The inducing molecules from WISH, L, and ME cells had molecular weights of 130,000, 90,000, and 68,000, respectively. Further purification, plus a confirmation of the glycoprotein nature of the inducer molecule, was shown by specifically binding and eluting L cell inducer bioactivity from a concanavalin A-Sepharose affinity column.

Full text

PDF
220

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BADER J. P. Production of interferon by chick embryo cells exposed to Rous sarcoma virus. Virology. 1962 Apr;16:436–443. doi: 10.1016/0042-6822(62)90224-6. [DOI] [PubMed] [Google Scholar]
  2. Beck J., Engler H., Brunner H., Kirchner H. Interferon production in cocultures between mouse spleen cells and tumor cells: possible role of mycoplasmas in interferon induction. J Immunol Methods. 1980;38(1-2):63–73. doi: 10.1016/0022-1759(80)90331-2. [DOI] [PubMed] [Google Scholar]
  3. Blalock J. E., Langford M. P., Georgiades J., Stanton G. J. Nonsensitized lymphocytes produce leukocyte interferon when cultured with foreign cells. Cell Immunol. 1979 Mar 1;43(1):197–201. doi: 10.1016/0008-8749(79)90163-1. [DOI] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of leucocytes from human blood. Further observations. Methylcellulose, dextran, and ficoll as erythrocyteaggregating agents. Scand J Clin Lab Invest Suppl. 1968;97:31–50. [PubMed] [Google Scholar]
  5. Campbell J. B., Grunberger T., Kochman M. A., White S. L. A microplaque reduction assay for human and mouse interferon. Can J Microbiol. 1975 Aug;21(8):1247–1253. doi: 10.1139/m75-186. [DOI] [PubMed] [Google Scholar]
  6. Ito Y., Nishiyama Y., Shimokata K., Nagata I., Takeyama H., Kunii A. The mechanism of interferon induction in mouse spleen cells stimulated with HVJ. Virology. 1978 Jul 1;88(1):128–137. doi: 10.1016/0042-6822(78)90116-2. [DOI] [PubMed] [Google Scholar]
  7. Ito Y., Nishiyama Y., Shimokata K., Takeyama H., Kunii A. Active component of HVJ (sendai virus) for interferon on induction in mice. Nature. 1978 Aug 24;274(5673):801–802. doi: 10.1038/274801a0. [DOI] [PubMed] [Google Scholar]
  8. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SINKOVICS J. G., HOWE C. D. GROWTH CHARACTERISTICS, VIRUS YIELD, AND INTERFERON ASSAY OF LEUKEMIC MOUSE SPLEEN TISSUE CULTURES. J Infect Dis. 1964 Oct;114:359–372. doi: 10.1093/infdis/114.4.359. [DOI] [PubMed] [Google Scholar]
  10. Trinchieri G., Santoli D., Dee R. R., Knowles B. B. Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation. J Exp Med. 1978 May 1;147(5):1299–1313. doi: 10.1084/jem.147.5.1299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Trinchieri G., Santoli D., Knowles B. B. Tumour cell lines induce interferon in human lymphocytes. Nature. 1977 Dec 15;270(5638):611–613. doi: 10.1038/270611a0. [DOI] [PubMed] [Google Scholar]
  12. Weigent D. A., Langford M. P., Smith E. M., Blalock J. E., Stanton G. J. Human B lymphocytes produce leukocyte interferon after interaction with foreign cells. Infect Immun. 1981 May;32(2):508–512. doi: 10.1128/iai.32.2.508-512.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES