Abstract
The ability of lipid A preparations from strains of Bacteroides, Fusobacterium, and Veillonella to inhibit the lipid A-anti-lipid A reaction in an enzyme-linked immunosorbent assay was tested. Anti-lipid A serum was prepared with lipid A from Salmonella minnesota R595, and lipid A from Escherichia coli EH100 was used as control antigen. Preparations from three of four different species of Bacteroides were unable to inhibit the anti-lipid A activity, whereas lipid A preparations from Fusobacterium and Veillonella strains inhibited 50% of the activity at 1 to 141 micrograms. One of the Bacteroides strains, Bacteroides oralis, showed a very weak inhibiting activity at the highest concentration used. The results confirm that Bacteroides species have a unique lipopolysaccharide structure, in contrast to other anaerobic genera which have a lipopolysaccharide structure similar to that of the Enterobacteriaceae.
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Selected References
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- Bishop D. G., Hewett M. J., Knox K. W. Biochemical studies on lipopolysaccharides of Veillonella. Eur J Biochem. 1971 Mar 11;19(2):169–175. doi: 10.1111/j.1432-1033.1971.tb01301.x. [DOI] [PubMed] [Google Scholar]
- Dahlén G., Hofstad T. Endotoxic activities of lipopolysaccharides of microorganisms isolated from an infected root canal in Macaca cynomolgus. Scand J Dent Res. 1977 May;85(4):272–278. doi: 10.1111/j.1600-0722.1977.tb00563.x. [DOI] [PubMed] [Google Scholar]
- Fabricius L., Dahlén G., Holm S. E., Möller A. J. Influence of combinations of oral bacteria on periapical tissues of monkeys. Scand J Dent Res. 1982 Jun;90(3):200–206. doi: 10.1111/j.1600-0722.1982.tb00728.x. [DOI] [PubMed] [Google Scholar]
- Finegold S. M. Taxonomy, enzymes, and clinical relevance of anaerobic bacteria. Rev Infect Dis. 1979 Mar-Apr;1(2):248–253. doi: 10.1093/clinids/1.2.248. [DOI] [PubMed] [Google Scholar]
- Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
- Galanos C. Physical state and biological activity of lipopolysaccharides. Toxicity and immunogenicity of the lipid A component. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):214–229. [PubMed] [Google Scholar]
- Gorbach V. I., Krasikova I. N., Lukyanov P. A., Razmakhnina O. Y., Solov'eva T. F., Ovodov Y. S. Structural studies on the immunodominant group of lipid A from lipopolysaccharide of Yersinia pseudotuberculosis. Eur J Biochem. 1979 Jul;98(1):83–86. doi: 10.1111/j.1432-1033.1979.tb13163.x. [DOI] [PubMed] [Google Scholar]
- Hase S., Hofstad T., Rietschel E. T. Chemical structure of the lipid A component of lipopolysaccharides from Fusobacterium nucleatum. J Bacteriol. 1977 Jan;129(1):9–14. doi: 10.1128/jb.129.1.9-14.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hofstad T., Kristoffersen T. Chemical characteristics of endotoxin from Bacteroides fragilis NCTC 9343. J Gen Microbiol. 1970 Apr;61(1):15–19. doi: 10.1099/00221287-61-1-15. [DOI] [PubMed] [Google Scholar]
- Hofstad T., Skaug N. Fatty acids and neutral sugars present in lipopolysaccharides isolated from Fusobacterium species. Acta Pathol Microbiol Scand B. 1980 Apr;88(2):115–120. doi: 10.1111/j.1699-0463.1980.tb02615.x. [DOI] [PubMed] [Google Scholar]
- Hofstad T. The distribution of heptose and 2-keto-3-deoxy-octonate in Bacteroidaceae. J Gen Microbiol. 1974 Dec;85(2):314–320. doi: 10.1099/00221287-85-2-314. [DOI] [PubMed] [Google Scholar]
- Johns M. A., Bruins S. C., McCabe W. R. Immunization with R mutants of Salmonella minnesota. II. Serological response to lipid A and the lipopolysaccharide of Re mutants. Infect Immun. 1977 Jul;17(1):9–15. doi: 10.1128/iai.17.1.9-15.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasper D. L. Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1976 Jul;134(1):59–66. doi: 10.1093/infdis/134.1.59. [DOI] [PubMed] [Google Scholar]
- Lugowski C., Romanowska E. Biological properties of lipid A from Shigella sonnei. Eur J Biochem. 1974 Oct 1;48(1):81–87. doi: 10.1111/j.1432-1033.1974.tb03745.x. [DOI] [PubMed] [Google Scholar]
- Mattsby-Baltzer I., Claësson I., Hanson L. A., Jodal U., Kaijser B., Lindberg U., Peterson H. Antibodies to lipid A during urinary tract infection. J Infect Dis. 1981 Oct;144(4):319–328. doi: 10.1093/infdis/144.4.319. [DOI] [PubMed] [Google Scholar]
- Mattsby-Baltzer I., Kaijser B. Lipid A and anti-lipid A. Infect Immun. 1979 Mar;23(3):758–763. doi: 10.1128/iai.23.3.758-763.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nygren H., Dahlén G., Nilsson L. A. Human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula. Infect Immun. 1979 Nov;26(2):391–396. doi: 10.1128/iai.26.2.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sveen K., Hofstad T., Milner K. C. Lethality for mice and chick embryos, pyrogenicity in rabbits and ability to gelate lysate from amoebocytes of Limulus polyphemus by lipopolysaccharides from Bacteroides, Fusobacterium and Veillonella. Acta Pathol Microbiol Scand B. 1977 Dec;85B(6):388–396. doi: 10.1111/j.1699-0463.1977.tb01994.x. [DOI] [PubMed] [Google Scholar]
- Sveen K. The capacity of lipopolysaccharides from bacteroides, fusobacterium and veillonella to produce skin inflammation and the local and generalized Shwartzman reaction in rabbits. J Periodontal Res. 1977 Sep;12(5):340–350. doi: 10.1111/j.1600-0765.1977.tb01525.x. [DOI] [PubMed] [Google Scholar]
- Wollenweber H. W., Rietschel E. T., Hofstad T., Weintraub A., Lindberg A. A. Nature, type of linkage, quantity, and absolute configuration of (3-hydroxy) fatty acids in lipopolysaccharides from Bacteroides fragilis NCTC 9343 and related strains. J Bacteriol. 1980 Dec;144(3):898–903. doi: 10.1128/jb.144.3.898-903.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
