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. 1983 Feb;39(2):505–513. doi: 10.1128/iai.39.2.505-513.1983

HeLa cell invasiveness and O antigen of Shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs.

N Okamura, T Nagai, R Nakaya, S Kondo, M Murakami, K Hisatsune
PMCID: PMC347979  PMID: 6187681

Abstract

Many rough mutants selected from isogenic smooth virulent and avirulent strains of Shigella flexneri were examined for virulence, using tissue culture infection and Sereny tests. Many of the rough mutants isolated from a virulent smooth strain were capable of penetrating tissue culture cells but incapable of producing a positive Sereny test. In contrast, we could not obtain from smooth avirulent strains any rough mutants capable of penetrating HeLa cells. Chemical analysis of lipopolysaccharide of some representative rough strains showed several patterns of sugar composition with a range of from Ra to Re chemotypes. There was no correlation between HeLa cell invasiveness and chemotypes of lipopolysaccharides, thus indicating little significance of oligosaccharides of the rough core as well as O antigens in the ability of S. flexneri to penetrate HeLa cells. When these invasive rough strains were given O antigen genes from a smooth avirulent Shigella Hfr strain, most of the transconjugants that expressed O antigens regained the ability to evoke keratoconjunctivitis in guinea pigs. We also examined the chromosomal loci of HeLa cell invasion by transferring carbohydrate fermentation genes of Escherichia coli K-12 Hfr and found two chromosomal loci, the rha and lac-gal regions, which control the ability to penetrate HeLa cells. These results suggested that O antigens and ability to penetrate tissue culture cells are independent and prerequisite attributes of virulence in Shigella flexneri to evoke keratoconjunctivitis in guinea pigs.

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Selected References

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  1. Adamus G., Mulczyk M., Witkowska D., Romanowska E. Protection against keratoconjunctivitis shigellosa induced by immunization with outer membrane proteins of Shigella spp. Infect Immun. 1980 Nov;30(2):321–324. doi: 10.1128/iai.30.2.321-324.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bagdian G., Dröge W., Kotelko K., Lüderitz O., Westphal O. Vorkommen zweier Heptosen in Lipopolysacchariden enterobakterieller Zellwände: L-Glycero-und D-Glycero-D-mannoheptose. Biochem Z. 1966 Mar 28;344(2):197–211. [PubMed] [Google Scholar]
  3. FALKOW S., SCHNEIDER H., BARON L. S., FORMAL S. B. VIRULENCE OF ESCHERICHIA-SHIGELLA GENETIC HYBRIDS FOR THE GUINEA PIG. J Bacteriol. 1963 Dec;86:1251–1258. doi: 10.1128/jb.86.6.1251-1258.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FORMAL S. B., LABREC E. H., KENT T. H., FALKOW S. ABORTIVE INTESTINAL INFECTION WITH AN ESCHERICHIA COLI-SHIGELLA FLEXNERI HYBRID STRAIN. J Bacteriol. 1965 May;89:1374–1382. doi: 10.1128/jb.89.5.1374-1382.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fensom A. H., Gray G. W. The chemical composition of the lipopolyacarideof Pseudomonas aeruginosa. Biochem J. 1969 Sep;114(2):185–196. doi: 10.1042/bj1140185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Formal S. B., Gemski P., Baron L. S., Labrec E. H. A Chromosomal Locus Which Controls the Ability of Shigella flexneri to Evoke Keratoconjunctivitis. Infect Immun. 1971 Jan;3(1):73–79. doi: 10.1128/iai.3.1.73-79.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Formal S. B., Gemski P., Baron L. S., Labrec E. H. Genetic Transfer of Shigella flexneri Antigens to Escherichia coli K-12. Infect Immun. 1970 Mar;1(3):279–287. doi: 10.1128/iai.1.3.279-287.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  9. Gemski P., Jr, Koeltzow D. E., Formal S. B. Phage conversion of Shigella flexneri group antigens. Infect Immun. 1975 Apr;11(4):685–691. doi: 10.1128/iai.11.4.685-691.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnston J. H., Johnston R. J., Simmons D. A. The immunochemistry of Shigella flexneri O-antigens. The biochemical basis of smooth to rough mutation. Biochem J. 1967 Oct;105(1):79–87. doi: 10.1042/bj1050079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lindberg A. A. Bacteriophage receptors. Annu Rev Microbiol. 1973;27:205–241. doi: 10.1146/annurev.mi.27.100173.001225. [DOI] [PubMed] [Google Scholar]
  13. Lüderitz O., Ruschmann E., Westphal O., Raff R., Wheat R. Occurrence of 3-amino-3,6-dideoxyhexoses in Salmonella and related bacteria. J Bacteriol. 1967 May;93(5):1681–1687. doi: 10.1128/jb.93.5.1681-1687.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lüderitz O., Staub A. M., Westphal O. Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966 Mar;30(1):192–255. doi: 10.1128/br.30.1.192-255.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okamura N., Nakaya R. Rough mutant of Shigella flexneri 2a that penetrates tissue culture cells but does not evoke keratoconjunctivitis in guinea pigs. Infect Immun. 1977 Jul;17(1):4–8. doi: 10.1128/iai.17.1.4-8.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sansonetti P. J., Kopecko D. J., Formal S. B. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. doi: 10.1128/iai.35.3.852-860.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WATKINS H. M. Some attributes of virulence in Shigella. Ann N Y Acad Sci. 1960 Nov 21;88:1167–1186. doi: 10.1111/j.1749-6632.1960.tb20107.x. [DOI] [PubMed] [Google Scholar]
  19. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]

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