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. 1983 Feb;39(2):970–973. doi: 10.1128/iai.39.2.970-973.1983

Plasmid Coding for Drug Resistance and Production of Heat-Labile and Heat-Stable Toxins Harbored by an Escherichia coli Strain of Human Origin

M Lourdes M Silva 1, Isabel C A Scaletsky 1, M Henriqueta L Reis 1,†, M Heloiza T Affonso 1,‡, Luiz R Trabulsi 1
PMCID: PMC348042  PMID: 6339395

Abstract

In a study of enterotoxigenic strains of Escherichia coli isolated from children with diarrhea in São Paulo, Brazil, a new enterotoxin and antibiotic resistance plasmid that carries heat-labile toxin, heat-stable toxin, and drug resistance genes was found. This is the first such plasmid to be found in a human strain of E. coli. The plasmid is nonconjugative, has a molecular weight of at least 54 × 106, and is mobilized by the R plasmid present in the host strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  3. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  4. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  5. Elwell L. P., Shipley P. L. Plasmid-mediated factors associated with virulence of bacteria to animals. Annu Rev Microbiol. 1980;34:465–496. doi: 10.1146/annurev.mi.34.100180.002341. [DOI] [PubMed] [Google Scholar]
  6. Gyles C. L., Barnum D. A. A heat-labile enterotoxin from strains of Eschericha coli enteropathogenic for pigs. J Infect Dis. 1969 Oct;120(4):419–426. doi: 10.1093/infdis/120.4.419. [DOI] [PubMed] [Google Scholar]
  7. Gyles C. L., Palchaudhuri S., Maas W. K. Naturally occurring plasmid carrying genes for enterotoxin production and drug resistance. Science. 1977 Oct 14;198(4313):198–199. doi: 10.1126/science.333581. [DOI] [PubMed] [Google Scholar]
  8. McConnell M. M., Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. Transposition of ampicillin resistance to an enterotoxin plasmid in an Escherichia coli strain of human origin. J Bacteriol. 1979 Aug;139(2):346–355. doi: 10.1128/jb.139.2.346-355.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reis M. H., Castro A. F., Toledo M. R., Trabulsi L. R. Production of heat-stable enterotoxin by the O128 serogroup of Escherichia coli. Infect Immun. 1979 Apr;24(1):289–290. doi: 10.1128/iai.24.1.289-290.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sack D. A., Sack R. B. Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture. Infect Immun. 1975 Feb;11(2):334–336. doi: 10.1128/iai.11.2.334-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sack R. B. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. doi: 10.1146/annurev.mi.29.100175.002001. [DOI] [PubMed] [Google Scholar]
  12. Silva M. L., Maas W. K., Gyles C. L. Isolation and characterization of enterotoxin-deficient mutants of Escherichia coli. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1384–1388. doi: 10.1073/pnas.75.3.1384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stieglitz H., Fonseca R., Olarte J., Kupersztoch-Portnoy Y. M. Linkage of heat-stable enterotoxin activity and ampicillin resistance in a plasmid isolated from an Escherichia coli strain of human origin. Infect Immun. 1980 Nov;30(2):617–620. doi: 10.1128/iai.30.2.617-620.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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