Abstract
Cryoprecipitation was observed in defibrinated serum from Schistosoma japonicum-infected rabbits. Ouchterlony immunodiffusion, sodium dodecyl sulfate-polyacrylamide gels, and anti-cryoprecipitate antiserum demonstrated the presence of immunoglobulin G (IgG), IgM, C3, fibrinogen, and an alpha-macroglobulin. Parasite antigen was not detected. IgG and IgM from pooled cryoprecipitates failed to react with each other in Ouchterlony immunodiffusion gels after separation by column chromatography. The data suggest that the IgG and IgM are not specifically complexed with each other but may simply be aggregates of altered proteins.
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- Bout D., Santoro F., Carlier Y., Bina J. C., Capron A. Circulating immune complexes in schistosomiasis. Immunology. 1977 Jul;33(1):17–22. [PMC free article] [PubMed] [Google Scholar]
- Brouet J. C., Clauvel J. P., Danon F., Klein M., Seligmann M. Biologic and clinical significance of cryoglobulins. A report of 86 cases. Am J Med. 1974 Nov;57(5):775–788. doi: 10.1016/0002-9343(74)90852-3. [DOI] [PubMed] [Google Scholar]
- Digeon M., Droz D., Noel L. H., Riza J., Rieumailhol C., Bach J. F., Santoro F., Capron A. The role of circulating immune complexes in the glomerular disease of experimental hepatosplenic schistosomiasis. Clin Exp Immunol. 1979 Mar;35(3):329–337. [PMC free article] [PubMed] [Google Scholar]
- Druet P., Letonturier P., Contet A., Mandet C. Cryoglobulinaemia in human renal diseases. A study of seventy-six cases. Clin Exp Immunol. 1973 Dec;15(4):483–496. [PMC free article] [PubMed] [Google Scholar]
- Grupe W. E. IgG-B1C cryoglobulins in acute glomerulonephritis. Pediatrics. 1968 Sep;42(3):474–482. [PubMed] [Google Scholar]
- Hanauer L. B., Christian C. L. Studies of cryoproteins in systemic lupus erythematosus. J Clin Invest. 1967 Mar;46(3):400–408. doi: 10.1172/JCI105541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones C. E., Rachford F. W., Ozcel M. A., Lewert R. M. Schistosoma japonicum: semiquantitative assessment of circulating immune complexes, serum C1q and C3, and their relationship to renal pathology and hepatic fibrosis in rabbits. Exp Parasitol. 1977 Jun;42(1):221–234. doi: 10.1016/0014-4894(77)90080-7. [DOI] [PubMed] [Google Scholar]
- McIntosh R. M., Kaufman D. B., Kulvinskas C., Grossman B. J. Cryoglobulins. I. Studies on the nature, incidence, and clinical significance of serum cryoproteins in glomerulonephritis. J Lab Clin Med. 1970 Apr;75(4):566–577. [PubMed] [Google Scholar]
- Meltzer M., Franklin E. C. Cryoglobulinemia--a study of twenty-nine patients. I. IgG and IgM cryoglobulins and factors affecting cryoprecipitability. Am J Med. 1966 Jun;40(6):828–836. doi: 10.1016/0002-9343(66)90199-9. [DOI] [PubMed] [Google Scholar]
- Meltzer M., Franklin E. C., Elias K., McCluskey R. T., Cooper N. Cryoglobulinemia--a clinical and laboratory study. II. Cryoglobulins with rheumatoid factor activity. Am J Med. 1966 Jun;40(6):837–856. doi: 10.1016/0002-9343(66)90200-2. [DOI] [PubMed] [Google Scholar]
- Robinson A., Lewert R. M., Spargo B. H. Immune complex glomerulonephritis and amyloidosis in Schistosoma japonicum infected rabbits. Trans R Soc Trop Med Hyg. 1982;76(2):214–226. doi: 10.1016/0035-9203(82)90279-6. [DOI] [PubMed] [Google Scholar]
- Smith M. D., Verroust P. J., Morel-Maroger L., Geniteau M., CouLaud J. P. A study of the presence of circulating immune complexes in schistosomiasis. Trans R Soc Trop Med Hyg. 1977;71(4):343–348. doi: 10.1016/0035-9203(77)90116-x. [DOI] [PubMed] [Google Scholar]
- Spear P. G., Roizman B. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J Virol. 1972 Jan;9(1):143–159. doi: 10.1128/jvi.9.1.143-159.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]