Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jun;40(3):1140–1145. doi: 10.1128/iai.40.3.1140-1145.1983

Binding of Todd-Hewitt broth antigens by Streptococcus mutans.

M W Stinson, C A Jones
PMCID: PMC348169  PMID: 6852915

Abstract

Streptococcus mutans 10449, grown in chemically defined culture medium, was tested for its ability to bind 3H-labeled Todd-Hewitt broth components (greater than 12,000 Mr). Maximum adsorption of radioactivity occurred within 5 min at room temperature, and cell-bound material was not completely removed by extended washing with buffer. Heat-killed, arsenate-inhibited, and viable bacteria bound similar quantities. Only 0.09% of the radioactivity in the preparation of high Mr Todd-Hewitt broth components was removed by absorption with excess numbers of S. mutans 10449 cells. Binding followed saturation kinetics and was competitively inhibited by unlabeled medium components, both the dialyzable and nondialyzable fractions. Other oral streptococci were also found to bind these complex medium components. Rabbit antiserum elicited to the high-molecular-weight Todd-Hewitt broth components reacted with monkey cardiac muscle and with S. mutans coated with medium components. Absorption of the anti-Todd-Hewitt broth serum with homogenized heart removed antibodies that reacted with Todd-Hewitt broth-coated S. mutans. Therefore, the tissue-specific antigens of this beef heart infusion medium that adsorb to S. mutans can interfere with the detection and characterization of antigens shared by these bacteria and animal tissues.

Full text

PDF
1140

Images in this article

1144Image
on p.1144

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alkan M., Ofek I., Beachey E. H. Adherence pharyngeal and skin strains of group A streptococci to human skin and oral epithelial cells. Infect Immun. 1977 Nov;18(2):555–557. doi: 10.1128/iai.18.2.555-557.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. Eibl H., Lands W. E. A new, sensitive determination of phosphate. Anal Biochem. 1969 Jul;30(1):51–57. doi: 10.1016/0003-2697(69)90372-8. [DOI] [PubMed] [Google Scholar]
  4. Ferretti J. J., Shea C., Humphrey M. W. Cross-reactivity of Streptococcus mutans antigens and human heart tissue. Infect Immun. 1980 Oct;30(1):69–73. doi: 10.1128/iai.30.1.69-73.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibbons R. J., Qureshi J. V. Selective binding of blood group-reactive salivary mucins by Streptococcus mutans and other oral organisms. Infect Immun. 1978 Dec;22(3):665–671. doi: 10.1128/iai.22.3.665-671.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibbons R. J., van Houte J. Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant. Infect Immun. 1971 Apr;3(4):567–573. doi: 10.1128/iai.3.4.567-573.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hamilton I. R., Phipps P. J., Ellwood D. C. Effect of growth rate and glucose concentration on the biochemical properties of Streptococcus mutans Ingbritt in continuous culture. Infect Immun. 1979 Dec;26(3):861–869. doi: 10.1128/iai.26.3.861-869.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardy L., Jacques N. A., Forester H., Campbell L. K., Knox K. W., Wicken A. J. Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production, and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture. Infect Immun. 1981 Jan;31(1):78–87. doi: 10.1128/iai.31.1.78-87.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  10. Hughes M., Machardy S. M., Sheppard A. J., Woods N. C. Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue. Infect Immun. 1980 Feb;27(2):576–588. doi: 10.1128/iai.27.2.576-588.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacques N. A., Hardy L., Knox K. W., Wicken A. J. Effect of growth conditions on the formation of extracellular lipoteichoic acid by Streptococcus mutans BHT. Infect Immun. 1979 Jul;25(1):75–84. doi: 10.1128/iai.25.1.75-84.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KAPLAN M. H. IMMUNOLOGIC RELATION OF STREPTOCOCCAL AND TISSUE ANTIGENS. I. PROPERTIES OF AN ANTIGEN IN CERTAIN STRAINS OF GROUP A STREPTOCOCCI EXHIBITING AN IMMUNOLOGIC CROSS-REACTION WITH HUMAN HEART TISSUE. J Immunol. 1963 Apr;90:595–606. [PubMed] [Google Scholar]
  13. Levine M. J., Herzberg M. C., Levine M. S., Ellison S. A., Stinson M. W., Li H. C., van Dyke T. Specificity of salivary-bacterial interactions: role of terminal sialic acid residues in the interaction of salivary glycoproteins with Streptococcus sanguis and Streptococcus mutans. Infect Immun. 1978 Jan;19(1):107–115. doi: 10.1128/iai.19.1.107-115.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ofek I., Beachey E. H., Jefferson W., Campbell G. L. Cell membrane-binding properties of group A streptococcal lipoteichoic acid. J Exp Med. 1975 May 1;141(5):990–1003. doi: 10.1084/jem.141.5.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RAPAPORT F. T., CHASE R. M., Jr HOMOGRAFT SENSITIVITY INDUCTION BY GROUP A STREPTOCOCCI. Science. 1964 Jul 24;145(3630):407–408. doi: 10.1126/science.145.3630.407. [DOI] [PubMed] [Google Scholar]
  16. Stinson M. W., Bergey E. J. Isolation of heart- and kidney-binding protein from group A streptococci. Infect Immun. 1982 Jan;35(1):335–342. doi: 10.1128/iai.35.1.335-342.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stinson M. W., Jinks D. C., Merrick J. M. Adherence of Streptococcus mutans and Streptococcus sanguis to salivary components bound to glass. Infect Immun. 1981 May;32(2):583–591. doi: 10.1128/iai.32.2.583-591.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stinson M. W., Levine M. J., Cavese J. M., Prakobphol A., Murray P. A., Tabak L. A., Reddy M. S. Adherence of Streptococcus sanguis to salivary mucin bound to glass. J Dent Res. 1982 Dec;61(12):1390–1393. doi: 10.1177/00220345820610120101. [DOI] [PubMed] [Google Scholar]
  19. Zabriskie J. B., Freimer E. H. An immunological relationship between the group. A streptococcus and mammalian muscle. J Exp Med. 1966 Oct 1;124(4):661–678. doi: 10.1084/jem.124.4.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van de Rijn I., Kessler R. E. Growth characteristics of group A streptococci in a new chemically defined medium. Infect Immun. 1980 Feb;27(2):444–448. doi: 10.1128/iai.27.2.444-448.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES