Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1983 Jun;40(3):1214–1217. doi: 10.1128/iai.40.3.1214-1217.1983

Cytopathogenicity of Naegleria fowleri for rat neuroblastoma cell cultures: scanning electron microscopy study.

F Marciano-Cabral, D T John
PMCID: PMC348179  PMID: 6852919

Abstract

Neuroblastoma cells were inoculated with Naegleria fowleri Lee and examined for cytopathology at various periods post-inoculation by scanning electron microscopy. By 18 h post-inoculation, approximately 50% of neuroblastoma cells were nonviable, as evidenced by trypan blue exclusion and light microscopic examination. This cytopathology resulted from piecemeal consumption of target cells mediated by a sucker apparatus extending from the surface of N. fowleri.

Full text

PDF
1214

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown T. Observations by immunofluorescence microscopy and electron microscopy on the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures. J Med Microbiol. 1979 Aug;12(3):363–371. doi: 10.1099/00222615-12-3-363. [DOI] [PubMed] [Google Scholar]
  2. Brown T. Observations by light microscopy on the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures. J Med Microbiol. 1978 Aug;11(3):249–259. doi: 10.1099/00222615-11-3-249. [DOI] [PubMed] [Google Scholar]
  3. Brunk U., Collins V. P., Arro E. The fixation, dehydration, drying and coating of cultured cells of SEM. J Microsc. 1981 Aug;123(Pt 2):121–131. doi: 10.1111/j.1365-2818.1981.tb01288.x. [DOI] [PubMed] [Google Scholar]
  4. Culbertson C. G., Ensminger P. W., Overton W. M. Pathogenic Naegleria sp.--study of a strain isolated from human cerebrospinal fluid. J Protozool. 1968 May;15(2):353–363. doi: 10.1111/j.1550-7408.1968.tb02136.x. [DOI] [PubMed] [Google Scholar]
  5. Cursons R. T., Brown T. J. Use of cell cultures as an indicator of pathogenicity of free-living amoebae. J Clin Pathol. 1978 Jan;31(1):1–11. doi: 10.1136/jcp.31.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duma R. J., Rosenblum W. I., McGehee R. F., Jones M. M., Nelson E. C. Primary amoebic meningoencephalitis caused by Naegleria. Two new cases, response to amphotericin B, and a review. Ann Intern Med. 1971 Jun;74(6):923–931. doi: 10.7326/0003-4819-74-6-923. [DOI] [PubMed] [Google Scholar]
  7. Lushbaugh W. B., Hofbauer A. F., Pittman F. E., Pittman J. C. Surface ultrastructure of Entamoeba histolytica; a study by high voltage transmission electron microscopy (HVTEM) and scanning electron microscopy (SEM). Arch Invest Med (Mex) 1978;9 (Suppl 1):191–202. [PubMed] [Google Scholar]
  8. Marciano-Cabral F. M., Patterson M., John D. T., Bradley S. G. Cytopathogenicity of Naegleria fowleri and Naegleria gruberi for established mammalian cell cultures. J Parasitol. 1982 Dec;68(6):1110–1116. [PubMed] [Google Scholar]
  9. Visvesvara G. S., Callaway C. S. Light and electron microsopic observations on the pathogenesis of Naegleria fowleri in mouse brain and tissue culture. J Protozool. 1974 May;21(2):239–250. doi: 10.1111/j.1550-7408.1974.tb03648.x. [DOI] [PubMed] [Google Scholar]
  10. Weik R. R., John D. T. Agitated mass cultivation of Naegleria fowleri. J Parasitol. 1977 Oct;63(5):868–871. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES