Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jan;77(1):294–298. doi: 10.1073/pnas.77.1.294

In vitro synthesis of the full-length complement of the negative-strand genome RNA of vesicular stomatitis virus.

D Testa, P K Chanda, A K Banerjee
PMCID: PMC348256  PMID: 6244552

Abstract

Under the normal conditions of in vitro RNA synthesis, the virion-associated RNA polymerase of vesicular stomatitis virus synthesizes five monocristronic mRNAs and a 48-nucleotide-long leader RNA that represents the exact 3'-terminal region of the genome RNA [Colonno, R. J. & Banerjee, A. K. (1978) Cell, 15, 93-101]. When the transcribing core was preincubated with ATP and CTP, reisolated, and then incubated in the presence of the beta, gamma imido analogue of ATP (AdoPP[NH]P) and the three normal ribonucleoside triphosphates, the full-length complementary strand of the genome RNA was synthesized in vitro. The results suggest that specific phosphorylated states of regulatory proteins may control transcription in vitro to generate the full-length plus strands.

Full text

PDF
294

Images in this article

296Image
on p.296

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G., Banerjee A. K. Sequential transcription of the genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 May;73(5):1504–1508. doi: 10.1073/pnas.73.5.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball L. A., White C. N. Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):442–446. doi: 10.1073/pnas.73.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Banerjee A. K., Moyer S. A., Rhodes D. P. Studies on the in vitro adenylation of RNA by vesicular stomatitis virus. Virology. 1974 Oct;61(2):547–558. doi: 10.1016/0042-6822(74)90289-x. [DOI] [PubMed] [Google Scholar]
  5. Batt-Humphries S., Simonsen C., Ehrenfeld E. Full-length viral RNA synthesized in vitro by vesicular stomatitis virus-infected HeLa cell extracts. Virology. 1979 Jul 15;96(1):88–99. doi: 10.1016/0042-6822(79)90175-2. [DOI] [PubMed] [Google Scholar]
  6. Breindl M., Holland J. J. Studies on the in vitro transcription and translation of vesicular stomatitis virus mRNA. Virology. 1976 Aug;73(1):106–118. doi: 10.1016/0042-6822(76)90065-9. [DOI] [PubMed] [Google Scholar]
  7. Clinton G. M., Burge B. W., Huang A. S. Effects of phosphorylation and pH on the association of NS protein with vesicular stomatitis virus cores. J Virol. 1978 Aug;27(2):340–346. doi: 10.1128/jvi.27.2.340-346.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  9. Emerson S. U., Yu Y. Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1975 Jun;15(6):1348–1356. doi: 10.1128/jvi.15.6.1348-1356.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moyer S. A., Banerjee A. K. Messenger RNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus. Cell. 1975 Jan;4(1):37–43. doi: 10.1016/0092-8674(75)90131-2. [DOI] [PubMed] [Google Scholar]
  11. Sokol F., Clark H. F. Phosphoproteins, structural components of rhabdoviruses. Virology. 1973 Mar;52(1):246–263. doi: 10.1016/0042-6822(73)90413-3. [DOI] [PubMed] [Google Scholar]
  12. Testa D., Banerjee A. K. Initiation of RNA synthesis in vitro by vesicular stomatitis virus. Role of ATP. J Biol Chem. 1979 Mar 25;254(6):2053–2058. [PubMed] [Google Scholar]
  13. Wertz G. W. Isolation of possible replicative intermediate structures from vesicular stomatitis virus-infected cells. Virology. 1978 Mar;85(1):271–285. doi: 10.1016/0042-6822(78)90431-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES