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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jan;77(1):644–648. doi: 10.1073/pnas.77.1.644

Early nerve-muscle synapses in vitro release transmitter over postsynaptic membrane having low acetylcholine sensitivity.

S A Cohen
PMCID: PMC348331  PMID: 6244572

Abstract

Functional nerve-muscle synapses form rapidly in cultures of embryonic chicken spinal cord and muscle cells. As early as 30 min after nerve processes first contact muscle fibers they are able to release stimulus-evoked neurotransmitter. This release was detected only after wave-form averaging because of the exceedingly low amplitude of the synaptic potentials. This small size was likely due to a postsynaptic effect, because the electrophysiologically assayed acetylcholine sensitivity of the synaptic muscle membrane was low and did not differ significantly from extrasynaptic levels. Transmitter release was elicited both from along the lengths of nerve processes and from active growth cones.

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Selected References

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  1. Anderson M. J., Cohen M. W. Nerve-induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells. J Physiol. 1977 Jul;268(3):757–773. doi: 10.1113/jphysiol.1977.sp011880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson M. J., Kidokoro Y., Gruener R. Correlation between acetylcholine receptor localization and spontaneous synaptic potentials in cultures of nerve and muscle. Brain Res. 1979 Apr 20;166(1):185–190. doi: 10.1016/0006-8993(79)90662-0. [DOI] [PubMed] [Google Scholar]
  3. Bennett M. R., McLachlan E. M., Taylor R. S. The formation of synapses in reinnervated mammalian striated muscle. J Physiol. 1973 Sep;233(3):481–500. doi: 10.1113/jphysiol.1973.sp010319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bennett M. R., Pettigrew A. G., Taylor R. S. The formation of synapses in reinnervated and cross-reinnervated adult avian muscle. J Physiol. 1973 Apr;230(2):331–357. doi: 10.1113/jphysiol.1973.sp010191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Betz W. Functional and non-functional contacts between ciliary neurones and muscle grown in vitro. J Physiol. 1976 Jan;254(1):75–86. doi: 10.1113/jphysiol.1976.sp011222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S. A., Fischbach G. D. Clusters of acetylcholine receptors located at identified nerve-muscle synapses in vitro. Dev Biol. 1977 Aug;59(1):24–38. doi: 10.1016/0012-1606(77)90237-8. [DOI] [PubMed] [Google Scholar]
  7. Dennis M. J., Miledi R. Characteristics of transmitter release at regenerating frog neuromuscular junctions. J Physiol. 1974 Jun;239(3):571–594. doi: 10.1113/jphysiol.1974.sp010583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dennis M. J., Miledi R. Non-transmitting neuromuscular junctions during an early stage of end-plate reinnervation. J Physiol. 1974 Jun;239(3):553–570. doi: 10.1113/jphysiol.1974.sp010582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Engelhardt J. K., Ishikawa K., Mori J., Shimabukuro Y. Passive electrical properties of cultured chick skeletal muscle: neurotrophic effect on sample distribution. Brain Res. 1977 Apr 22;126(1):172–175. doi: 10.1016/0006-8993(77)90225-6. [DOI] [PubMed] [Google Scholar]
  10. Fertuck H. C., Salpeter M. M. Quantitation of junctional and extrajunctional acetylcholine receptors by electron microscope autoradiography after 125I-alpha-bungarotoxin binding at mouse neuromuscular junctions. J Cell Biol. 1976 Apr;69(1):144–158. doi: 10.1083/jcb.69.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fischbach G. D., Nameroff M., Nelson P. G. Electrical properties of chick skeletal muscle fibers developing in cell culture. J Cell Physiol. 1971 Oct;78(2):289–299. doi: 10.1002/jcp.1040780218. [DOI] [PubMed] [Google Scholar]
  12. Fischbach G. D. Synapse formation between dissociated nerve and muscle cells in low density cell cultures. Dev Biol. 1972 Jun;28(2):407–429. doi: 10.1016/0012-1606(72)90023-1. [DOI] [PubMed] [Google Scholar]
  13. Hauser S. E., Cohen S. A. An isolated current monitor for iontophoresis. ISA Trans. 1976;15(3):253–257. [PubMed] [Google Scholar]
  14. Kuffler S. W., Yoshikami D. The distribution of acetylcholine sensitivity at the post-synaptic membrane of vertebrate skeletal twitch muscles: iontophoretic mapping in the micron range. J Physiol. 1975 Jan;244(3):703–730. doi: 10.1113/jphysiol.1975.sp010821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Locke S., Solomon H. C. Relation of resting potential of rat gastrocnemius and soleus muscles to innervation, activity, and the Na-K pump. J Exp Zool. 1967 Dec;166(3):377–386. doi: 10.1002/jez.1401660310. [DOI] [PubMed] [Google Scholar]
  16. MILEDI R. Properties of regenerating neuromuscular synapses in the frog. J Physiol. 1960 Nov;154:190–205. doi: 10.1113/jphysiol.1960.sp006573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McArdle J. J., Albuquerque E. X. A study of the reinnervation of fast and slow mammalian muscles. J Gen Physiol. 1973 Jan;61(1):1–23. doi: 10.1085/jgp.61.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nelson P. G., Lux H. D. Some electrical measurements of motoneuron parameters. Biophys J. 1970 Jan;10(1):55–73. doi: 10.1016/S0006-3495(70)86285-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Puro D. G., De Mello F. G., Nirenberg M. Synapse turnover: the formation and termination of transient synapses. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4977–4981. doi: 10.1073/pnas.74.11.4977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Redfern P. A. Neuromuscular transmission in new-born rats. J Physiol. 1970 Aug;209(3):701–709. doi: 10.1113/jphysiol.1970.sp009187. [DOI] [PMC free article] [PubMed] [Google Scholar]

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