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. 1980 Feb;77(2):822–826. doi: 10.1073/pnas.77.2.822

Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase

Setsuko O Jolly 1, Lawrence Bogorad 1
PMCID: PMC348373  PMID: 16592779

Abstract

Zea mays chloroplast DNA-dependent RNA polymerase in vitro preferentially transcribes maize chloroplast DNA sequences incorporated in cloned chimeric bacterial plasmids. Preferential transcription is dependent on the presence of a 27.5-kilodalton polypeptide, the S factor, which has been purified from maize chloroplasts, and also on the template's being in the supercoiled form.

Keywords: S factor, plasmids, supercoiled DNA

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Selected References

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