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. 1980 Feb;77(2):862–865. doi: 10.1073/pnas.77.2.862

Phospholipid methylation: a biochemical signal modulating lymphocyte mitogenesis.

F Hirata, S Toyoshima, J Axelrod, M J Waxdal
PMCID: PMC348381  PMID: 6965797

Abstract

Phospholipid methylation in murine T lymphocytes but not B cells was stimulated by mitogenic lectins such as concanavalin A and phytohemagglutinin, and the methylation was then returned to the control level by the concomitant activation of phospholipase A2. A parallelism between dose-response curves of concanavalin A for phospholipid methylation and thymidine incorporation was found. Inhibition of either synthesis or degradation of methylated phospholipids resulted in a decrease in the thymidine incorporation. Although prostaglandins such as the E and F series were the main products of arachidonic acid released by phospholipase A2 activation, inhibition of synthesis of these compounds by indomethacin did not reduce the thymidine incorporation significantly. These results suggest that the mitogenesis of murine T lymphocytes is triggered by the activation of both phospholipid methyltransferase(s) and phospholipase A2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bona C., Robert-Gero M., Lederer E. Inhibition of mitogen induced blastogenesis by 5'-deoxy-5'-S-iso-butyl adenosine. Biochem Biophys Res Commun. 1976 May 17;70(2):622–629. doi: 10.1016/0006-291x(76)91093-7. [DOI] [PubMed] [Google Scholar]
  2. Chiang P. K., Cantoni G. L., Bader J. P., Shannon W. M., Thomas H. J., Montgomery J. A. Adenosylhomocysteine hydrolase inhibitors: synthesis of 5'-deoxy-5'-(isobutylthio)-3-deazaadenosine and its effect on Rous sarcoma virus and Gross murine leukemia virus. Biochem Biophys Res Commun. 1978 May 30;82(2):417–423. doi: 10.1016/0006-291x(78)90892-6. [DOI] [PubMed] [Google Scholar]
  3. Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
  4. Hirata F., Axelrod J. Enzymatic methylation of phosphatidylethanolamine increases erythrocyte membrane fluidity. Nature. 1978 Sep 21;275(5677):219–220. doi: 10.1038/275219a0. [DOI] [PubMed] [Google Scholar]
  5. Hirata F., Axelrod J. Enzymatic synthesis and rapid translocation of phosphatidylcholine by two methyltransferases in erythrocyte membranes. Proc Natl Acad Sci U S A. 1978 May;75(5):2348–2352. doi: 10.1073/pnas.75.5.2348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirata F., Strittmatter W. J., Axelrod J. beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling. Proc Natl Acad Sci U S A. 1979 Jan;76(1):368–372. doi: 10.1073/pnas.76.1.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Knudsen B. B., Dissing J. Adenosine deaminase deficiency in a child with severe combined immunodeficiency. Clin Genet. 1973;4(4):344–347. doi: 10.1111/j.1399-0004.1973.tb01929.x. [DOI] [PubMed] [Google Scholar]
  8. Kredich N. M., Martin D. V., Jr Role of S-adenosylhomocysteine in adenosinemediated toxicity in cultured mouse T lymphoma cells. Cell. 1977 Dec;12(4):931–938. doi: 10.1016/0092-8674(77)90157-x. [DOI] [PubMed] [Google Scholar]
  9. Shier W. T., Baldwin J. H., Nilsen-Hamilton M., Hamilton R. T., Thanassi N. M. Regulation of guanylate and adenylate cyclase activities by lysolecithin. Proc Natl Acad Sci U S A. 1976 May;73(5):1586–1590. doi: 10.1073/pnas.73.5.1586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Toyoshima S., Osawa T. Lectins from Wistaria floribunda seeds and their effect on membrane fluidity of human peripheral lymphocytes. J Biol Chem. 1975 Mar 10;250(5):1655–1660. [PubMed] [Google Scholar]
  11. Webb D. R., Nowowiejski I. Mitogen-induced changes in lymphocyte prostaglandin levels: a signal for the induction of suppressor cell activity. Cell Immunol. 1978 Nov;41(1):72–85. doi: 10.1016/s0008-8749(78)80029-x. [DOI] [PubMed] [Google Scholar]
  12. Zimmerman T. P., Wolberg G., Duncan G. S. Inhibition of lymphocyte-mediated cytolysis by 3-deazaadenosine: evidence for a methylation reaction essential to cytolysis. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6220–6224. doi: 10.1073/pnas.75.12.6220. [DOI] [PMC free article] [PubMed] [Google Scholar]

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