Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Feb;77(2):885–889. doi: 10.1073/pnas.77.2.885

Reversible dissociation of linker histone from chromatin with preservation of internucleosomal repeat.

J Allan, D Z Staynov, H Gould
PMCID: PMC348386  PMID: 6928686

Abstract

Procedures are described for the dissociation of histones H1 and H5 from chicken reticulocyte chromatin without disruption of the native core histone-DNA complex. The comparative properties of native and depleted chromatin with respect to sedimentation, thermal denaturation, and sensitivity to nuclease digestion have been studied. The changes in these properties resulting from removal of the linker histones are fully reversed when histone H5 is added back to the depleted chromatin.

Full text

PDF
885

Images in this article

888Image
on p.888

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan J., Fey S. J., Cowling G. J., Gould H. J., Maryanka D. Pathway-dependent reconstitution of chromatin structure from separated constituents. J Biol Chem. 1979 Nov 10;254(21):11061–11065. [PubMed] [Google Scholar]
  2. Bellard M., Oudet P., Germond J. E., Chambon P. Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976 Nov 15;70(2):543–553. doi: 10.1111/j.1432-1033.1976.tb11046.x. [DOI] [PubMed] [Google Scholar]
  3. Billett M. A., Barry J. M. Role of histones in chromatin condensation. Eur J Biochem. 1974 Dec 2;49(3):477–484. doi: 10.1111/j.1432-1033.1974.tb03852.x. [DOI] [PubMed] [Google Scholar]
  4. Bina-Stein M., Simpson R. T. Specific folding and contraction of DNA by histones H3 and H4. Cell. 1977 Jul;11(3):609–618. doi: 10.1016/0092-8674(77)90078-2. [DOI] [PubMed] [Google Scholar]
  5. Bonner W. M., Stedman J. D. Histone 1 is proximal to histone 2A and to A24. Proc Natl Acad Sci U S A. 1979 May;76(5):2190–2194. doi: 10.1073/pnas.76.5.2190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Boseley P. G., Bradbury E. M., Butler-Browne G. S., Carpenter B. G., Stephens R. M. Physical studies of chromatin. The recombination of histones with DNA. Eur J Biochem. 1976 Feb 2;62(1):21–31. doi: 10.1111/j.1432-1033.1976.tb10093.x. [DOI] [PubMed] [Google Scholar]
  7. Bradbury E. M., Danby S. E., Rattle H. W., Giancotti V. Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. Histone H1 in chromatin and in H1 - DNA complexes. Eur J Biochem. 1975 Sep 1;57(1):97–105. doi: 10.1111/j.1432-1033.1975.tb02280.x. [DOI] [PubMed] [Google Scholar]
  8. Camerini-Otero R. D., Sollner-Webb B., Felsenfeld G. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. Cell. 1976 Jul;8(3):333–347. doi: 10.1016/0092-8674(76)90145-8. [DOI] [PubMed] [Google Scholar]
  9. Davies H. G., Murray A. B., Walmsley M. E. Electron-microscope observations on the organization of the nucleus in chicken erythrocytes and a superunit thread hypothesis for chromosome structure. J Cell Sci. 1974 Nov;16(2):261–299. doi: 10.1242/jcs.16.2.261. [DOI] [PubMed] [Google Scholar]
  10. Finch J. T., Klug A. Solenoidal model for superstructure in chromatin. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897–1901. doi: 10.1073/pnas.73.6.1897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fulmer A. W., Fasman G. D. Analysis of chromatin reconstitutiion. Biochemistry. 1979 Feb 20;18(4):659–668. doi: 10.1021/bi00571a017. [DOI] [PubMed] [Google Scholar]
  12. Germond J. E., Bellard M., Oudet P., Chambon P. Stability of nucleosomes in native and reconstituted chromatins. Nucleic Acids Res. 1976 Nov;3(11):3173–3192. doi: 10.1093/nar/3.11.3173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gould H. J., Maryanka D., Fey S. J., Cowling G. J., Allan J. The assay of globin gene transcription in reconstituted chromatin. Methods Cell Biol. 1978;19:387–422. doi: 10.1016/s0091-679x(08)60038-2. [DOI] [PubMed] [Google Scholar]
  14. Ilyin Y. V., Varshavsky A. Y., Mickelsaar U. N., Georgiev G. P. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA. Eur J Biochem. 1971 Sep 24;22(2):235–245. doi: 10.1111/j.1432-1033.1971.tb01537.x. [DOI] [PubMed] [Google Scholar]
  15. Kornberg R. D. Chromatin structure: a repeating unit of histones and DNA. Science. 1974 May 24;184(4139):868–871. doi: 10.1126/science.184.4139.868. [DOI] [PubMed] [Google Scholar]
  16. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Lawson G. M., Cole R. D. Selective displacement of histone H1 from whole HeLa nuclei: effect on chromatin structure in situ as probed by micrococcal nuclease. Biochemistry. 1979 May 29;18(11):2160–2166. doi: 10.1021/bi00578a005. [DOI] [PubMed] [Google Scholar]
  20. Li H. J., Bonner J. Interaction of histone half-molecules with deoxyribonucleic acid. Biochemistry. 1971 Apr 13;10(8):1461–1470. doi: 10.1021/bi00784a030. [DOI] [PubMed] [Google Scholar]
  21. McKnight G. S. A colorimetric method for the determination of submicrogram quantities of protein. Anal Biochem. 1977 Mar;78(1):86–92. doi: 10.1016/0003-2697(77)90011-2. [DOI] [PubMed] [Google Scholar]
  22. Moss T., Stephens R. M., Crane-Robinson C., Bradbury E. M. A nucleosome-like structure containing DNA and the arginine-rich histones H3 and H4. Nucleic Acids Res. 1977 Jul;4(7):2477–2485. doi: 10.1093/nar/4.7.2477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Müller U., Zentgraf H., Eicken I., Keller W. Higher order structure of simian virus 40 chromatin. Science. 1978 Aug 4;201(4354):406–415. doi: 10.1126/science.208155. [DOI] [PubMed] [Google Scholar]
  24. NEELIN J. M., CALLAHAN P. X., LAMB D. C., MURRAY K. THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI. Can J Biochem. 1964 Dec;42:1743–1752. doi: 10.1139/o64-185. [DOI] [PubMed] [Google Scholar]
  25. Noll M., Kornberg R. D. Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977 Jan 25;109(3):393–404. doi: 10.1016/s0022-2836(77)80019-3. [DOI] [PubMed] [Google Scholar]
  26. Oudet P., Gross-Bellard M., Chambon P. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell. 1975 Apr;4(4):281–300. doi: 10.1016/0092-8674(75)90149-x. [DOI] [PubMed] [Google Scholar]
  27. Pospelov V. A., Svetlikova S. B., Vorob'ev V. I. Nucleosome packing in chromatin as revealed by nuclease digestion. Nucleic Acids Res. 1979 Jan;6(1):399–419. doi: 10.1093/nar/6.1.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Renz M., Nehls P., Hozier J. Involvement of histone H1 in the organization of the chromosome fiber. Proc Natl Acad Sci U S A. 1977 May;74(5):1879–1883. doi: 10.1073/pnas.74.5.1879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shaw B. R., Herman T. M., Kovacic R. T., Beaudreau G. S., Van Holde K. E. Analysis of subunit organization in chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1976 Feb;73(2):505–509. doi: 10.1073/pnas.73.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Simon R. H., Camerini-Otero R. D., Felsenfeld G. An octamer of histones H3 and H4 forms a compact complex with DNA of nucleosome size. Nucleic Acids Res. 1978 Dec;5(12):4805–4818. doi: 10.1093/nar/5.12.4805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sollner-Webb B., Camerini-Otero R. D., Felsenfeld G. Chromatin structure as probed by nucleases and proteases: evidence for the central role of histones H3 and H4. Cell. 1976 Sep;9(1):179–193. doi: 10.1016/0092-8674(76)90063-5. [DOI] [PubMed] [Google Scholar]
  32. Spadafora C., Oudet P., Chambon P. The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones. Nucleic Acids Res. 1978 Oct;5(10):3479–3489. doi: 10.1093/nar/5.10.3479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Staynov D. Z. Thermal denaturation profiles and the structure of chromatin. Nature. 1976 Dec 9;264(5586):522–525. doi: 10.1038/264522a0. [DOI] [PubMed] [Google Scholar]
  34. Steinmetz M., Streeck R. E., Zachau H. G. Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin. Eur J Biochem. 1978 Feb;83(2):615–628. doi: 10.1111/j.1432-1033.1978.tb12131.x. [DOI] [PubMed] [Google Scholar]
  35. Tatchell K., Van Holde K. E. Reconstitution of chromatin core particles. Biochemistry. 1977 Nov 29;16(24):5295–5303. doi: 10.1021/bi00643a021. [DOI] [PubMed] [Google Scholar]
  36. Thoma F., Koller T. Influence of histone H1 on chromatin structure. Cell. 1977 Sep;12(1):101–107. doi: 10.1016/0092-8674(77)90188-x. [DOI] [PubMed] [Google Scholar]
  37. Varshavsky A. J., Bakayev V. V., Georgiev G. P. Heterogeneity of chromatin subunits in vitro and location of histone H1. Nucleic Acids Res. 1976 Feb;3(2):477–492. doi: 10.1093/nar/3.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Weischet W. O., Allen J. R., Riedel G., Van Holde K. E. The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease. Nucleic Acids Res. 1979;6(5):1843–1862. doi: 10.1093/nar/6.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Weissbach A., Poonian M. Nucleic acids attached to solid matrices. Methods Enzymol. 1974;34:463–475. doi: 10.1016/s0076-6879(74)34057-8. [DOI] [PubMed] [Google Scholar]
  40. Whitlock J. P., Jr, Simpson R. T. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes. Biochemistry. 1976 Jul 27;15(15):3307–3314. doi: 10.1021/bi00660a022. [DOI] [PubMed] [Google Scholar]
  41. Woodcock C. L. Reconstitution of chromatin subunits. Science. 1977 Mar 25;195(4284):1350–1352. doi: 10.1126/science.841333. [DOI] [PubMed] [Google Scholar]
  42. Worcel A., Benyajati C. Higher order coiling of DNA in chromatin. Cell. 1977 Sep;12(1):83–100. doi: 10.1016/0092-8674(77)90187-8. [DOI] [PubMed] [Google Scholar]
  43. Yaneva M., Tasheva B., Dessev G. Nuclease digestion of reconstituted chromatin. FEBS Lett. 1976 Nov;70(1):67–70. doi: 10.1016/0014-5793(76)80727-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES