Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Feb;77(2):1063–1067. doi: 10.1073/pnas.77.2.1063

Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.

B W Glickman, M Radman
PMCID: PMC348424  PMID: 6987663

Abstract

Our approach to the isolation of DNA mismatch-correction-deficient mutants was based upon the isolation of 2-aminopurine-resistant second-site revertants of Escherichia coli dam- mutants. We isolated such second-site revertants which, when separated from the dam- mutation, have a mutator character of their own. These new mutators all mapped at three known mutator loci, mutH, mutL, and mutS, which exhibit the same mutagenic spectrum as the dam- mutator: increased levels of base substitution and frameshift mutations. The mutator potencies of double and triple mut- mutants suggest that these mutators are involved in the same general mismatch-repair pathway. All these mutations result in a hyper-recombination phenotype, but in four-factor crosses among lambda phages, a specific loss of intragenic recombination (Pam3 X Pam80) was found in mutL and mutS mutants, as would be predicted from the postulated role of mismatch correction in gene conversion and high negative interference phenomena.

Full text

PDF
1063

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal S. S., Dube D. K., Loeb L. A. On the fidelity of DNA replication. Accuracy of Escherichia coli DNA polymerase I. J Biol Chem. 1979 Jan 10;254(1):101–106. [PubMed] [Google Scholar]
  2. Amati P, Meselson M. Localized Negative Interference in Bacteriophage. Genetics. 1965 Mar;51(3):369–379. doi: 10.1093/genetics/51.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bale A., d'Alarcao M., Marinus M. G. Characterization of DNA adenine methylation mutants of Escherichia coli K12. Mutat Res. 1979 Feb;59(2):157–165. doi: 10.1016/0027-5107(79)90153-2. [DOI] [PubMed] [Google Scholar]
  4. Cox E. C. Bacterial mutator genes and the control of spontaneous mutation. Annu Rev Genet. 1976;10:135–156. doi: 10.1146/annurev.ge.10.120176.001031. [DOI] [PubMed] [Google Scholar]
  5. Fincham J. R., Holliday R. An explanation of fine structure map expansion in terms of excision repair. Mol Gen Genet. 1970;109(4):309–322. doi: 10.1007/BF00267701. [DOI] [PubMed] [Google Scholar]
  6. Glickman B. W. Spontaneous mutagenesis in Escherichia coli strains lacking 6-methyladenine residues in their DNA: an altered mutational spectrum in dam- mutants. Mutat Res. 1979 Jul;61(2):153–162. doi: 10.1016/0027-5107(79)90122-2. [DOI] [PubMed] [Google Scholar]
  7. Glickman B., van den Elsen P., Radman M. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance. Mol Gen Genet. 1978 Jul 25;163(3):307–312. doi: 10.1007/BF00271960. [DOI] [PubMed] [Google Scholar]
  8. Glickman W., Guijt N., Morand P. The genetic characterization of lexB32, lexB33 and lexB35 mutations of Escherichia coli: location and complementation pattern for UV resistance. Mol Gen Genet. 1977 Nov 29;157(1):83–89. doi: 10.1007/BF00268690. [DOI] [PubMed] [Google Scholar]
  9. Lacks S., Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977 Jul;114(1):153–168. doi: 10.1016/0022-2836(77)90289-3. [DOI] [PubMed] [Google Scholar]
  10. Lambertsson A. G. The ribosomal proteins of drosophila melanogaster. IV. Characterization by two-dimensional gel electrophoresis of the ribosomal proteins from nine postembryonic developmental stages. Mol Gen Genet. 1975 Aug 5;139(2):133–144. [PubMed] [Google Scholar]
  11. Marinus M. G. Adenine methylation of Okazaki fragments in Escherichia coli. J Bacteriol. 1976 Dec;128(3):853–854. doi: 10.1128/jb.128.3.853-854.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marinus M. G. Location of DNA methylation genes on the Escherichia coli K-12 genetic map. Mol Gen Genet. 1973 Dec 14;127(1):47–55. doi: 10.1007/BF00267782. [DOI] [PubMed] [Google Scholar]
  13. Marinus M. G., Morris N. R. Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res. 1975 Apr;28(1):15–26. doi: 10.1016/0027-5107(75)90309-7. [DOI] [PubMed] [Google Scholar]
  14. Radding C. M. Genetic recombination: strand transfer and mismatch repair. Annu Rev Biochem. 1978;47:847–880. doi: 10.1146/annurev.bi.47.070178.004215. [DOI] [PubMed] [Google Scholar]
  15. Radman M., Villani G., Boiteux S., Kinsella A. R., Glickman B. W., Spadari S. Replicational fidelity: mechanisms of mutation avoidance and mutation fixation. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):937–946. doi: 10.1101/sqb.1979.043.01.103. [DOI] [PubMed] [Google Scholar]
  16. Rydberg B. Bromouracil mutagenesis and mismatch repair in mutator strains of Escherichia coli. Mutat Res. 1978 Oct;52(1):11–24. doi: 10.1016/0027-5107(78)90091-x. [DOI] [PubMed] [Google Scholar]
  17. Rydberg B. Bromouracil mutagenesis in Escherichia coli evidence for involvement of mismatch repair. Mol Gen Genet. 1977 Mar 28;152(1):19–28. doi: 10.1007/BF00264935. [DOI] [PubMed] [Google Scholar]
  18. Tiraby J. G., Fox M. S. Marker discrimination in transformation and mutation of pneumococcus. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3541–3545. doi: 10.1073/pnas.70.12.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WHITEHOUSE H. L., HASTINGS P. J. THE ANALYSIS OF GENETIC RECOMBINATION ON THE POLARON HYBRID DNA MODEL. Genet Res. 1965 Feb;6:27–92. doi: 10.1017/s0016672300003955. [DOI] [PubMed] [Google Scholar]
  20. Wagner R., Jr, Meselson M. Repair tracts in mismatched DNA heteroduplexes. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135–4139. doi: 10.1073/pnas.73.11.4135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. White R. L., Fox M. S. Genetic consequences of transfection with heterduplex bacteriophage lambda DNA. Mol Gen Genet. 1975 Nov 24;141(2):163–171. doi: 10.1007/BF00267681. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES