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. Author manuscript; available in PMC: 2013 Sep 28.
Published in final edited form as: Oncogene. 2012 May 21;32(13):1616–1625. doi: 10.1038/onc.2012.193

Figure 4.

Figure 4

HOTAIR overexpression in pancreatic cancer cells. (A) MiaPaCa2 and Panc28 cells were transfected with HOTAIR expression plasmid, and the effects on cell invasion were determined in a Boyden chamber assay. Results are expressed as means ± SE for 3 separate determinations and significantly (p < 0.05) enhanced invasion is indicated. Cells were transfected with HOTAIR expression plasmid and expression of genes regulated by HOTAIR overexpression in MDA-MB-231 cells (20) (B) and the HOTAIR repressed genes investigated in Figure 3A (C) were determined by real time PCR. Cells were transfected with HOTAIR expression plasmid, and effects on MiaPaCa2 and Panc28 cell proliferation (D) and cell cycle progression (E) are indicated. Results are expressed as means ± SE for 3 replicate determinations and significant (p < 0.05) induction or inhibition is indicated (*).