Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Feb;77(2):1139–1143. doi: 10.1073/pnas.77.2.1139

Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mononuclear cells.

M K Hoffmann
PMCID: PMC348440  PMID: 6444723

Abstract

Culture conditions suitable for specific sensitization of human peripheral blood lymphocytes were established. Production of antibody to sheep erythrocytes and to the 2,4,6-trinitrophenyl group conjugated to burro erythrocytes was investigated in terms of cross-sensitization, feedback control by antibody to the immunogen, and regulation by helper and suppressor T cells. Generation of antigen-specific antibody-forming cells was found to be restricted to a narrow concentration range of cultured peripheral blood lymphocytes. The data suggest that antibody formation is limited by suppressor T cells in high-cell-density cultures and by low frequencies of helper T cells in low-cell-density cultures.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dosch H-M, Gelfand E. W. Generation of human plaque-forming cells in culture: tissue distribution, antigenic and cellular requirements. J Immunol. 1977 Jan;118(1):302–308. [PubMed] [Google Scholar]
  2. Dutton R. W. Suppressor T cells. Transplant Rev. 1975;26:39–55. doi: 10.1111/j.1600-065x.1975.tb00174.x. [DOI] [PubMed] [Google Scholar]
  3. Fauci A. S., Pratt K. R. Activation of human B lymphocytes. I. Direct plaque-forming cell assay for the measurement of polyclonal activation and antigenic stimulation of human B lymphocytes. J Exp Med. 1976 Sep 1;144(3):674–684. doi: 10.1084/jem.144.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finelt M., Hoffmann M. K. A human monocyte function test: release of B-cell differentiation factor (BDF). Clin Immunol Immunopathol. 1979 Mar;12(3):281–288. doi: 10.1016/0090-1229(79)90031-x. [DOI] [PubMed] [Google Scholar]
  5. Hoffman M. K., Schmidt D., Oettgen H. F. Production of antibody to sheep red blood cells by human tonsil cells in vitro. Nature. 1973 Jun 15;243(5407):408–410. doi: 10.1038/243408a0. [DOI] [PubMed] [Google Scholar]
  6. Hoffmann M. K., Koenig S., Mittler R. S., Oettgen H. F., Ralph P., Galanos C., Hammerling U. Macrophage factor controlling differentiation of B cells. J Immunol. 1979 Feb;122(2):497–502. [PubMed] [Google Scholar]
  7. Hoffmann M. K., Watson J. Helper T cell-replacing factors secreted by thymus-derived cells and macrophages: cellular requirements for B cell activation and synergistic properties. J Immunol. 1979 Apr;122(4):1371–1375. [PubMed] [Google Scholar]
  8. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  9. Kettman J., Dutton R. W. An in vitro primary immune response to 2,4,6-trinitrophenyl substituted erythrocytes: response against carrier and hapten. J Immunol. 1970 Jun;104(6):1558–1561. [PubMed] [Google Scholar]
  10. Luzzati A. L., Hengartner H., Schreier M. H. Induction of plaque-forming cells in cultured human lymphocytes by combined action of antigen and EB virus. Nature. 1977 Sep 29;269(5627):419–420. doi: 10.1038/269419a0. [DOI] [PubMed] [Google Scholar]
  11. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moretta L., Ferrarini M., Mingari M. C., Moretta A., Webb S. R. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976 Dec;117(6):2171–2174. [PubMed] [Google Scholar]
  13. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Muchmore A. V., Koski I., Dooley N., Blaese R. M. Artifactual plaque formation in vitro and in vivo to passive transfer of specific antibody. J Immunol. 1976 Apr;116(4):1016–1019. [PubMed] [Google Scholar]
  15. Pickel K., Hoffmann M. K. Suppressor T cells arising in mice undergoing a graft-vs-host response. J Immunol. 1977 Feb;118(2):653–656. [PubMed] [Google Scholar]
  16. Pickel K., Hoffmann M. K. The Ly phenotype of suppressor T cells arising in mice subjected to a graft-versus-host reaction. J Exp Med. 1977 May 1;145(5):1169–1175. doi: 10.1084/jem.145.5.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sakane T., Green I. Protein A from Staphylococcus aureus-a mitogen for human T lymphocytes and B lymphocytes but not L lymphocytes. J Immunol. 1978 Jan;120(1):302–311. [PubMed] [Google Scholar]
  18. Swain S. L., Trefts P. E., Tse H. Y., Dutton R. W. The significance of T-B collaboration across haplotype barriers. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):597–609. doi: 10.1101/sqb.1977.041.01.069. [DOI] [PubMed] [Google Scholar]
  19. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES