Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Feb;77(2):1164–1168. doi: 10.1073/pnas.77.2.1164

Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.

Y Reisner, S Ikehara, M Z Hodes, R A Good
PMCID: PMC348445  PMID: 6965796

Abstract

Mouse spleen cells sequentially agglutinated by soybean agglutinin (SBA) and peanut agglutinin (PNA) previously had been shown to be sufficiently depleted of graft-versus-host activity to allow reconstitution of lethally irradiated allogeneic recipient mice. We have now tested the extent of T-cell depletion in this cell fraction by various in vitro assays, including cytotoxicity testing with anti-Thy-1 antiserum, mitogenic response to the T-cell mitogens concanavalin A and phytohemagglutinin and allogeneic responsiveness in the mixed lymphocytes culture assay. By these criteria the SBA+, PNA+ spleen fraction, used previously in the in vivo experiments, was found to possess about 1% T-cell contamination. The slight contamination with T cells previously found in the singly agglutinated SBA fraction can be removed by a second fractionation with SBA, thus eliminating the possibility that a minor T-cell subpopulation bears receptors for SBA. Finally, we demonstrated that the twice-agglutinated fraction, by SBA and PNA or by SBA alone, contains a significant number of prothymocytes, thereby indicating that mouse prothymocytes bear receptors for both SBA and PNA. The implication of these findings to bone marrow transplantation in humans is discussed.

Full text

PDF
1164

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eisenthal A., Nachtigal D., Feldman M. Studies of allospecific suppressor T lymphocytes induced and assayed in culture. Transplant Proc. 1979 Mar;11(1):904–906. [PubMed] [Google Scholar]
  2. Gordon Julius A., Blumberg Shmaryahu, Lis Halina, Sharon Nathan. Purification of soybean agglutinin by affinity chromatography On sepharose-N-epsilon-aminocaproyl-beta-D-galactopyranosylamine. FEBS Lett. 1972 Aug 1;24(2):193–196. doi: 10.1016/0014-5793(72)80765-8. [DOI] [PubMed] [Google Scholar]
  3. Haran-Ghera N., Rubio N., Leef F., Goldstein G. Characteristics of preleukemia cells induced in mice. Cell Immunol. 1978 May;37(2):308–314. doi: 10.1016/0008-8749(78)90199-5. [DOI] [PubMed] [Google Scholar]
  4. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  5. London J., Berrih S., Bach J. F. Peanut agglutinin. I. A new tool for studying T lymphocyte subpopulations. J Immunol. 1978 Aug;121(2):438–443. [PubMed] [Google Scholar]
  6. Lotan R., Skutelsky E., Danon D., Sharon N. The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem. 1975 Nov 10;250(21):8518–8523. [PubMed] [Google Scholar]
  7. Rabinovich H., Umiel T., Reisner Y., Sharon N., Globerson A. Characterization of embryonic liver suppressor cells by peanut agglutinin. Cell Immunol. 1979 Oct;47(2):347–355. doi: 10.1016/0008-8749(79)90344-7. [DOI] [PubMed] [Google Scholar]
  8. Reisner Y., Biniaminov M., Rosenthal E., Sharon N., Ramot B. Interaction of peanut agglutinin with normal human lymphocytes and with leukemic cells. Proc Natl Acad Sci U S A. 1979 Jan;76(1):447–451. doi: 10.1073/pnas.76.1.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reisner Y., Gachelin G., Dubois P., Nicolas J. F., Sharon N., Jacob F. Interaction of peanut agglutinin, a lectin specific for nonreducing terminal D-galactosyl residues, with embryonal carcinoma cells. Dev Biol. 1977 Nov;61(1):20–27. doi: 10.1016/0012-1606(77)90338-4. [DOI] [PubMed] [Google Scholar]
  10. Reisner Y., Itzicovitch L., Meshorer A., Sharon N. Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2933–2936. doi: 10.1073/pnas.75.6.2933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reisner Y., Linker-Israeli M., Sharon N. Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin. Cell Immunol. 1976 Jul;25(1):129–134. doi: 10.1016/0008-8749(76)90103-9. [DOI] [PubMed] [Google Scholar]
  12. Reisner Y., Ravid A., Sharon N. Use of soybean agglutinin for the separation of mouse B and T lymphocytes. Biochem Biophys Res Commun. 1976 Oct 18;72(4):1585–1591. doi: 10.1016/s0006-291x(76)80195-7. [DOI] [PubMed] [Google Scholar]
  13. TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES