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. 1980 Mar;77(3):1270–1273. doi: 10.1073/pnas.77.3.1270

Arogenate (pretyrosine) is an obligatory intermediate of L-tyrosine biosynthesis: confirmation in a microbial mutant.

A M Fazel, J R Bowen, R A Jensen
PMCID: PMC348475  PMID: 6929482

Abstract

Wild-type Brevibacterium flavum has been shown to possess arogenate dehydrogenase activity and to lack prephenate dehydrogenase, thereby providing presumptive evidence that arogenate (previously named "pretyrosine") is an obligatory intermediate of L-tyrosine biosynthesis. A similar enzymological pattern has been discerned in extracts made from wild-type cultures of various species of cyanobacteria. Application of rigorous molecular genetic criteria in confirmation of the exclusive role of arogenate in L-tyrosine synthesis was made possible by the isolation of an auxotrophic mutant exhibiting a nutritional requirement for L-tyrosine. The mutant was found to lack activity for arogenate dehydrogenase and to accumulate substantial amounts of arogenate behind the mutant block during starvation for L-tyrosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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