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. 1980 Mar;77(3):1301–1305. doi: 10.1073/pnas.77.3.1301

A human protein related to the major envelope protein of murine mammary tumor virus: identification and characterization.

A S Dion, D C Farwell, A A Pomenti, A J Girardi
PMCID: PMC348482  PMID: 6154929

Abstract

A human milk protein was isolated to apparent homogeneity and shown to be immunologically related to gp55, the major envelope glycoprotein of murine mammary tumor virus. Through the development of ultrasensitive protocols, which have wide applicability, immunological relatedness was corroborated by the demonstration of homologous protein sequences between the human and viral proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adair W. S., Jurivich D., Goodenough U. W. Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels. J Cell Biol. 1978 Oct;79(1):281–285. doi: 10.1083/jcb.79.1.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Axel R., Schlom J., Spiegelman S. Presence in human breast cancer of RNA homologous to mouse mammary tumour virus RNA. Nature. 1972 Jan 7;235(5332):32–36. doi: 10.1038/235032a0. [DOI] [PubMed] [Google Scholar]
  3. Black M. M., Zachrau R. E., Shore B., Dion A. S., Leis H. P., Jr Cellular immunity to autologous breast cancer and RIII-murine mammary tumor virus preparations. Cancer Res. 1978 Jul;38(7):2068–2076. [PubMed] [Google Scholar]
  4. Blair P. B. Immunology of the mouse mammary tumor virus: comparison of the antigenicity of mammary tumor virus obtained from several strains of mice. Cancer Res. 1970 Mar;30(3):625–631. [PubMed] [Google Scholar]
  5. Charney J., Pullinger B. D., Moore D. H. Development of an infectivity assay for mouse mammary-tumor virus. J Natl Cancer Inst. 1969 Dec;43(6):1289–1296. [PubMed] [Google Scholar]
  6. Dion A. S., Vaidya A. B., Fout G. S. Cation preferences for poly(rC)-oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates. Cancer Res. 1974 Dec;34(12):3509–3515. [PubMed] [Google Scholar]
  7. Dion A. S., Williams C. J., Pomenti A. A. The major structural proteins of murine mammary tumor virus: techniques for isolation. Anal Biochem. 1977 Sep;82(1):18–28. doi: 10.1016/0003-2697(77)90129-4. [DOI] [PubMed] [Google Scholar]
  8. Haber J. E., Koshland D. E., Jr An evaluation of the relatedness of proteins based on comparison of amino acid sequences. J Mol Biol. 1970 Jun 28;50(3):617–639. doi: 10.1016/0022-2836(70)90089-6. [DOI] [PubMed] [Google Scholar]
  9. Kantor J. A., Lee Y. H., Chirikjian J. G., Feller W. F. DNA polymerase with characteristics of reverse transcriptase purified from human milk. Science. 1979 May 4;204(4392):511–513. doi: 10.1126/science.86209. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Mesa-Tejada R., Keydar I., Ramanarayanan M., Ohno T., Fenoglio C., Spiegelman S. Detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1529–1533. doi: 10.1073/pnas.75.3.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moore D. H., Charney J., Kramarsky B., Lasfargues E. Y., Sarkar N. H., Brennan M. J., Burrows J. H., Sirsat S. M., Paymaster J. C., Vaidya A. B. Search for a human breast cancer virus. Nature. 1971 Feb 26;229(5287):611–614. doi: 10.1038/229611a0. [DOI] [PubMed] [Google Scholar]
  14. Ohno T., Mesa-Tejada R., Keydar I., Ramanarayanan M., Bausch J., Spiegelman S. Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1979 May;76(5):2460–2464. doi: 10.1073/pnas.76.5.2460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olden K., Yamada K. M. Direct detection of antigens in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem. 1977 Apr;78(2):483–490. doi: 10.1016/0003-2697(77)90108-7. [DOI] [PubMed] [Google Scholar]
  16. Sarkar N. H., Charney J., Dion A. S., Moore D. H. Effect of human milk on the mouse mammary tumor virus. Cancer Res. 1973 Mar;33(3):626–629. [PubMed] [Google Scholar]
  17. Sarkar N. H., Dion A. S. Polypeptides of the mouse mammary tumor virus. I. Characterization of two group-specific antigens. Virology. 1975 Apr;64(2):471–491. doi: 10.1016/0042-6822(75)90125-7. [DOI] [PubMed] [Google Scholar]
  18. Vaidya A. B., Black M. M., Dion A. S., Moore D. H. Homology between human breast tumour RNA and mouse mammary tumour virus genome. Nature. 1974 Jun 7;249(457):565–567. doi: 10.1038/249565a0. [DOI] [PubMed] [Google Scholar]
  19. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]
  20. Weiner A. M., Platt T., Weber K. Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis. J Biol Chem. 1972 May 25;247(10):3242–3251. [PubMed] [Google Scholar]
  21. Yang N. S., McGrath C. M., Furmanski P. Presence of a mouse mammary tumor virus-related antigen in human breast carcinoma cells and its absence from normal mammary epithelial cells. J Natl Cancer Inst. 1978 Nov;61(5):1205–1208. doi: 10.1093/jnci/61.5.1205. [DOI] [PubMed] [Google Scholar]
  22. Zachrau R. E., Black M. M., Dion A. S., Shore B., Williams C. J., Leis H. P., Jr Specificity of the simultaneous cell-mediated immune reactivity to RIII murine mammary tumor virus glycoprotein 55 and human breast cancer tissues. Cancer Res. 1978 Oct;38(10):3414–3420. [PubMed] [Google Scholar]

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