Figure 6.

DNA and viability analysis of the cytosolic transfer-positive UCB-MNC. (A) To exclude MSC transdifferentiation and MSC-UCB fusion as the reasons for the presence of CD45⁺ GFP⁺ cells, VNTR using PCR amplification at human locus D1S80 was carried out on the extracted DNA of the sorted CD45⁺ GFP⁺ cells. Lane 1, 1 kbp DNA ladder. Lane 2, GFP expressing ES-MSC. Lane 3, non-co-cultured UCB-MNC. Lane 4, sorted CD45⁺ GFP⁺ cells obtained after co-culturing for 3 days with GFP ES-MSC. Lane 5, sorted CD45⁺ cells obtained after co-culturing for 3 days with GFP ES-MSC. As shown, the band for CD45⁺ GFP⁺ cells (lane 4) corresponds with that of UCB-MNC only (lane 3). (B) Percentage of adherent CD45⁺ GFP⁺ UCB-MNC that are Annexin V^- when co-cultured with GFP ES-MSC over a 3–day time–course. Data represent mean±SEM from three independent experiments.