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. 1980 Mar;77(3):1617–1621. doi: 10.1073/pnas.77.3.1617

Humans have antibodies capable of recognizing oncoviral glycoproteins: demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus.

M Barbacid, D Bolognesi, S A Aaronson
PMCID: PMC348548  PMID: 6154936

Abstract

There is controversy in the literature concerning the presence in humans of antibodies directed against the envelope glycoproteins of known oncoviruses. In the present report, we show that antibodies capable of precipitating a wide variety of oncoviral glycoproteins can be demonstrated under certain assay conditions. Substances as diverse as normal components of serum, extracts of bacteria, and even nonprotein molecules such as glycogen also shared the oncoviral glycoprotein determinants recognized by normal human sera. It was found that immunoprecipitation of a given viral glycoprotein by human sera was entirely dependent upon the cell in which the virus was grown. Human sera specifically did not recognize glycoproteins purified from oncoviruses grown in human or higher primate cells. These findings not only demonstrate that the antibodies were directed against cellular rather than the virus-coded antigenic determinants but also exclude the possibility that this immune response was elicited as a consequence of oncovirus exposure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Stephenson J. R. Endogenous type-C RNA viruses of mammalian cells. Biochim Biophys Acta. 1976 Dec 23;458(4):323–354. doi: 10.1016/0304-419x(76)90006-8. [DOI] [PubMed] [Google Scholar]
  2. Aoki T., Walling M. J., Bushar G. S., Liu M., Hsu K. C. Natural antibodies in sera from healthy humans to antigens on surfaces of type C RNA viruses and cells from primates. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2491–2495. doi: 10.1073/pnas.73.7.2491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbacid M., Stephenson J. R., Aaronson S. A. gag Gene of mammalian type-C RNA tumour viruses. Nature. 1976 Aug 12;262(5569):554–559. doi: 10.1038/262554a0. [DOI] [PubMed] [Google Scholar]
  4. Bishop J. M. Retroviruses. Annu Rev Biochem. 1978;47:35–88. doi: 10.1146/annurev.bi.47.070178.000343. [DOI] [PubMed] [Google Scholar]
  5. Charman H. P., Kim N., White M., Gilden R. V. Failure to detect, in human sera, antibodies cross-reactive with group-specific antigens of murine leukemia virus. J Natl Cancer Inst. 1974 May;52(5):1409–1413. doi: 10.1093/jnci/52.5.1409. [DOI] [PubMed] [Google Scholar]
  6. Charman H. P., Kim N., White M., Marquardt H., Gilden R. V., Kawakami T. Natural and experimentally induced antibodies to defined mammalian type-C virus proteins in primates. J Natl Cancer Inst. 1975 Dec;55(6):1419–1424. doi: 10.1093/jnci/55.6.1419. [DOI] [PubMed] [Google Scholar]
  7. Charman H. P., Rahman R., White M. H., Kim N., Gilden R. V. Radioimmunoassay for the major structural protein of Mason-Pfizer monkey virus: Attempts to detect the presence of antigen or antibody in humans. Int J Cancer. 1977 Apr 15;19(4):498–504. doi: 10.1002/ijc.2910190410. [DOI] [PubMed] [Google Scholar]
  8. Devare S. G., Hanson R. E., Jr, Stephenson J. R. Primate retroviruses: envelope glycoproteins of endogenous type C and type D viruses possess common interspecies antigenic determinants. J Virol. 1978 May;26(2):316–324. doi: 10.1128/jvi.26.2.316-324.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardner M. B., Brown J. C., Charman H. P., Stephenson J. R., Rongey R. W., Hauser D. E., Diegmann F., Howard E., Dworsky R., Gilden R. V. FeLV epidemiology in Los Angeles cats: Appraisal of detection methods. Int J Cancer. 1977 Apr 15;19(4):581–589. doi: 10.1002/ijc.2910190420. [DOI] [PubMed] [Google Scholar]
  11. Hersh E. M., Gutterman J. U., Mavligit G., Gschwind C. R., Freireich E. J., Levine P. H., Plata E. J. Human immune response to active immunization with Rauscher leukemia virus. I. Cell-mediated and cell-associated immunity. J Natl Cancer Inst. 1974 Aug;53(2):317–325. doi: 10.1093/jnci/53.2.317. [DOI] [PubMed] [Google Scholar]
  12. Hino S., Stephenson J. R., Aaronson S. A. Antigenic determinants of the 70,000 molecular weight glycoprotein of woolly monkey type C RNA virus. J Immunol. 1975 Oct;115(4):922–927. [PubMed] [Google Scholar]
  13. Hino S., Stephenson J. R., Aaronson S. A. Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. J Virol. 1976 Jun;18(3):933–941. doi: 10.1128/jvi.18.3.933-941.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hirsch M. S., Kelly A. P., Chapin D. S., Fuller T. C., Black P. H., Kurth R. Immunity to antigens associated with primate C-type oncoviruses in pregnant women. Science. 1978 Mar 24;199(4335):1337–1340. doi: 10.1126/science.204010. [DOI] [PubMed] [Google Scholar]
  15. Ihle J. N., Denny T. P., Bolognesi D. P. Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice. J Virol. 1976 Mar;17(3):727–736. doi: 10.1128/jvi.17.3.727-736.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krakower J. M., Aaronson S. A. Seroepidemiologic assessment of feline leukaemia virus infection risk for man. Nature. 1978 Jun 8;273(5662):463–464. doi: 10.1038/273463a0. [DOI] [PubMed] [Google Scholar]
  17. Krakower J. M., Tronick S. R., Gallagher R. E., Gallo R. C., Aaronson S. A. Antigenic characterization of a new gibbon ape leukemia virus isolate: seroepidemiologic assessment of an outbreak of gibbon leukemia. Int J Cancer. 1978 Dec;22(6):715–720. doi: 10.1002/ijc.2910220613. [DOI] [PubMed] [Google Scholar]
  18. Kurth R., Huesgen A., Katz F., Löwer J. Comparison of radioimmunoprecipitation assays for the detection of human anti-tumor virus antibodies. J Immunol Methods. 1979;30(4):355–366. doi: 10.1016/0022-1759(79)90018-8. [DOI] [PubMed] [Google Scholar]
  19. Kurth R., Mikschy U. Human antibodies reactive with purified envelope antigens of primate type C tumor viruses. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5692–5696. doi: 10.1073/pnas.75.11.5692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kurth R., Schmitt C. Human humoral antibodies specific for primate C-type viral antigens. Med Microbiol Immunol. 1977;164(1-3):167–177. doi: 10.1007/BF02121312. [DOI] [PubMed] [Google Scholar]
  21. Kurth R., Teich N. M., Weiss R., Oliver R. T. Natural human antibodies reactive with primate type-C viral antigens. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1237–1241. doi: 10.1073/pnas.74.3.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  23. McSharry J. J., Ledda C. A., Freiman H. J., Choppin P. W. Biological properties of the VSV glycoprotein. II. Effects of the host cell and of the glycoprotein carbohydrate composition on hemagglutination. Virology. 1978 Jan;84(1):183–188. doi: 10.1016/0042-6822(78)90230-1. [DOI] [PubMed] [Google Scholar]
  24. Nakamura K., Compans R. W. Glycopeptide components of influenza viral glycoproteins. Virology. 1978 May 15;86(2):432–442. doi: 10.1016/0042-6822(78)90083-1. [DOI] [PubMed] [Google Scholar]
  25. Parham P., Sehgal P. K., Brodsky F. M. Anti-HLA-A,B,C monoclonal antibodies with no alloantigenic specificity in humans define polymorphisms in other primate species. Nature. 1979 Jun 14;279(5714):639–641. doi: 10.1038/279639a0. [DOI] [PubMed] [Google Scholar]
  26. Prochownik E. V., Kirsten W. H. Inhibition of reverse transcriptases of primate type C viruses by 7S immunoglobulin from patients with leukaemia. Nature. 1976 Mar 4;260(5546):64–67. doi: 10.1038/260064a0. [DOI] [PubMed] [Google Scholar]
  27. Schlesinger S., Gottlieb C., Feil P., Gelb N., Kornfeld S. Growth of enveloped RNA viruses in a line of chinese hamster ovary cells with deficient N-acetylglucosaminyltransferase activity. J Virol. 1975 Jan;17(1):239–246. doi: 10.1128/jvi.17.1.239-246.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Snyder H. W., Jr, Fleissner E. Specificity of human antibodies to oncovirus glycoproteins: recognition of antigen by natural antibodies directed against carbohydrate structures. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1622–1626. doi: 10.1073/pnas.77.3.1622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Snyder H. W., Jr, Fox M. Characterization of a fetal calf serum-derived molecule reactive with human natural antibodies: its occurrence in tissue culture-grown type C RNA viruses. J Immunol. 1978 Feb;120(2):646–651. [PubMed] [Google Scholar]
  30. Snyder H. W., Jr, Pincus T., Fleissner E. Specificities of human immunoglobulins reactive with antigens in preparations of several mammalian type-C viruses. Virology. 1976 Nov;75(1):60–73. doi: 10.1016/0042-6822(76)90007-6. [DOI] [PubMed] [Google Scholar]
  31. Stephenson J. R., Aaronson S. A. Search for antigens and antibodies crossreactive with type C viruses of the woolly monkeys and gibbon ape in animal models and in humans. Proc Natl Acad Sci U S A. 1976 May;73(5):1725–1729. doi: 10.1073/pnas.73.5.1725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stephenson J. R., Essex M., Hino S., Hardy W. D., Jr, Aaronson S. A. Feline oncornavirus-associated cell-membrane antigen (FOCMA): distinction between FOCMA and the major virion glycoprotein. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1219–1223. doi: 10.1073/pnas.74.3.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Stephenson J. R., Hino S., Garrett E. W., Aaronson S. A. Immunological cross reactivity of Mason-Pfizer monkey virus with type C RNA viruses endogenous to primates. Nature. 1976 Jun 17;261(5561):609–611. doi: 10.1038/261609a0. [DOI] [PubMed] [Google Scholar]
  34. Strand M., August J. T. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen. J Biol Chem. 1973 Aug 25;248(16):5627–5633. [PubMed] [Google Scholar]
  35. Thiel H. J., Beug H., Graf T., Schwarz H., Schäfer W., Bergholz C., Deinhardt F. Studies of simian sarcoma and simian sarcoma-associated virus. II. Isolation of the major viral glycoprotein, properties of this component and its specific antiserum. Virology. 1978 Oct 15;90(2):360–365. doi: 10.1016/0042-6822(78)90320-3. [DOI] [PubMed] [Google Scholar]

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