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. 1980 Apr;77(4):1867–1871. doi: 10.1073/pnas.77.4.1867

Translocation of phospholipids from the inner to the outer membrane of Escherichia coli.

A M Donohue-Rolfe, M Schaechter
PMCID: PMC348609  PMID: 6246510

Abstract

The translocation of lipids from the inner to the outer membrane of Escherichia coli has been investigated by pulse-chase experiments. After a pulse with [2-3H]glycerol, the specific activity of the newly synthesized [3H]phosphatidylethanolamine was 5 times greater in the inner than in the outer membrane. During the chase, [3H]phosphatidylethanolamine was translocated to the outer membrane. At 37 degrees C, the half-life for translocation was 2.8 min. This rate was not influenced by alteration in the cellular growth rate at 37 degrees C. Altering the cellular growth temperature had a pronounced effect on the rate of phosphatidylethanolamine translocation. Energy inhibitors that deplete the protonmotive force markedly inhibited the translocation. Translocation was not affected by inhibitors of ATP, protein, or lipid synthesis. Phosphatidylglycerol and cardiolipin are transfocated very rapidly, with half-lives shorter than 30 sec.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bayer M. E. Areas of adhesion between wall and membrane of Escherichia coli. J Gen Microbiol. 1968 Oct;53(3):395–404. doi: 10.1099/00221287-53-3-395. [DOI] [PubMed] [Google Scholar]
  3. Bayer M. E., Starkey T. W. The adsorption of bacteriophage phi X174 and its interaction with Escherichia coli; a kinetic and morphological study. Virology. 1972 Jul;49(1):236–256. doi: 10.1016/s0042-6822(72)80026-6. [DOI] [PubMed] [Google Scholar]
  4. Bell R. M., Mavis R. D., Osborn M. J., Vagelos P. R. Enzymes of phospholipid metabolism: localization in the cytoplasmic and outer membrane of the cell envelope of Escherichia coli and Salmonella typhimurium. Biochim Biophys Acta. 1971 Dec 3;249(2):628–635. doi: 10.1016/0005-2736(71)90144-1. [DOI] [PubMed] [Google Scholar]
  5. Cole H. A., Wimpenny J. W., Hughes D. E. The ATP pool in Escherichia coli. I. Measurement of the pool using modified luciferase assay. Biochim Biophys Acta. 1967;143(3):445–453. doi: 10.1016/0005-2728(67)90050-3. [DOI] [PubMed] [Google Scholar]
  6. Harold F. M. Conservation and transformation of energy by bacterial membranes. Bacteriol Rev. 1972 Jun;36(2):172–230. doi: 10.1128/br.36.2.172-230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hirschberg C. B., Kennedy E. P. Mechanism of the enzymatic synthesis of cardiolipin in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Mar;69(3):648–651. doi: 10.1073/pnas.69.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones N. C., Osborn M. J. Translocation of phospholipids between the outer and inner membranes of Salmonella typhimurium. J Biol Chem. 1977 Oct 25;252(20):7405–7412. [PubMed] [Google Scholar]
  9. Klein W. L., Boyer P. D. Energization of active transport by Escherichia coli. J Biol Chem. 1972 Nov 25;247(22):7257–7265. [PubMed] [Google Scholar]
  10. Kornberg R. D., McConnell H. M. Inside-outside transitions of phospholipids in vesicle membranes. Biochemistry. 1971 Mar 30;10(7):1111–1120. doi: 10.1021/bi00783a003. [DOI] [PubMed] [Google Scholar]
  11. Langley K. E., Kennedy E. P. Energetics of rapid transmembrane movement and of compositional asymmetry of phosphatidylethanolamine in membranes of Bacillus megaterium. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6245–6249. doi: 10.1073/pnas.76.12.6245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leduc M., Schaechter M. Size of the lipid precursor pool in Escherichia coli. J Bacteriol. 1978 Feb;133(2):1038–1038. doi: 10.1128/jb.133.2.1038-1038.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lin E. C. Glycerol dissimilation and its regulation in bacteria. Annu Rev Microbiol. 1976;30:535–578. doi: 10.1146/annurev.mi.30.100176.002535. [DOI] [PubMed] [Google Scholar]
  14. Mühlradt P. F., Menzel J., Golecki J. R., Speth V. Outer membrane of salmonella. Sites of export of newly synthesised lipopolysaccharide on the bacterial surface. Eur J Biochem. 1973 Jun 15;35(3):471–481. doi: 10.1111/j.1432-1033.1973.tb02861.x. [DOI] [PubMed] [Google Scholar]
  15. Omura S. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev. 1976 Sep;40(3):681–697. doi: 10.1128/br.40.3.681-697.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  17. Osborn M. J., Gander J. E., Parisi E. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Site of synthesis of lipopolysaccharide. J Biol Chem. 1972 Jun 25;247(12):3973–3986. [PubMed] [Google Scholar]
  18. Osborn M. J., Rick P. D., Lehmann V., Rupprecht E., Singh M. Structure and biogenesis of the cell envelope of gram-negative bacteria. Ann N Y Acad Sci. 1974 May 10;235(0):52–65. doi: 10.1111/j.1749-6632.1974.tb43256.x. [DOI] [PubMed] [Google Scholar]
  19. Pudek M. R., Bragg P. D. Reaction of cyanide with cytochrome d in respiratory particles from exponential phase Escherichia coli. FEBS Lett. 1975 Feb 1;50(2):111–113. doi: 10.1016/0014-5793(75)80468-6. [DOI] [PubMed] [Google Scholar]
  20. Rosen B. P. Restoration of active transport in an Mg2+-adenosine triphosphatase-deficient mutant of Escherichia coli. J Bacteriol. 1973 Dec;116(3):1124–1129. doi: 10.1128/jb.116.3.1124-1129.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rothman J. E., Dawidowicz E. A. Asymmetric exchange of vesicle phospholipids catalyzed by the phosphatidylcholine exhange protein. Measurement of inside--outside transitions. Biochemistry. 1975 Jul;14(13):2809–2816. doi: 10.1021/bi00684a004. [DOI] [PubMed] [Google Scholar]
  22. Rothman J. E., Kennedy E. P. Rapid transmembrane movement of newly synthesized phospholipids during membrane assembly. Proc Natl Acad Sci U S A. 1977 May;74(5):1821–1825. doi: 10.1073/pnas.74.5.1821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schnaitman C. A. Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis. J Bacteriol. 1970 Nov;104(2):882–889. doi: 10.1128/jb.104.2.882-889.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sinensky M. Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Feb;71(2):522–525. doi: 10.1073/pnas.71.2.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wais A. C., Goldberg E. B. Growth and transformation of phage T4 in Escherichia coli B-4, Salmonella, Aerobacter, Proteus, and Serratia. Virology. 1969 Oct;39(2):153–161. doi: 10.1016/0042-6822(69)90035-x. [DOI] [PubMed] [Google Scholar]
  26. White D. A., Albright F. R., Lennarz W. J., Schnaitman C. A. Distribution of phospholipid-synthesizing enzymes in the wall and membrane subfractions of the envelope of Escherichia coli. Biochim Biophys Acta. 1971 Dec 3;249(2):636–642. doi: 10.1016/0005-2736(71)90145-3. [DOI] [PubMed] [Google Scholar]

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