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. 1980 Apr;77(4):1907–1911. doi: 10.1073/pnas.77.4.1907

Limited DNase I nicking as a probe of gene conformation.

M Zasloff, R D Camerini-Otero
PMCID: PMC348618  PMID: 6929527

Abstract

We have extended the use of pancreatic DNase I as a probe of chromatin structure by exploring the accessibility of an active gene to the introduction of the first single-stranded nick. We show by a target analysis that the beta-globin gene is about 25-fold more sensitive to single-site nicking than is an average sequence in the chicken erythrocyte nucleus or the nontranscribed albumin gene. The sites of initial DNase I nicking are shown to cluster within the transcribed sequence of the beta-globin gene.

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Selected References

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