Abstract
Two species of H4 histone mRNA were isolated from the polysomes of S phase HeLa S3 cells. Electrophoresis under denaturing and nondenaturing conditions indicates that the two H4 mRNAs differ in size. Both mRNAs translate H4 histones in vitro, lack poly(A) at their 3' termini, and are capped at their 5' termini. Polyacrylamide gel electrophoresis and tryptic peptide analysis suggest that the polypeptides synthesized by the two mRNAs are indistinguishable.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- Elgin S. C., Weintraub H. Chromosomal proteins and chromatin structure. Annu Rev Biochem. 1975;44:725–774. doi: 10.1146/annurev.bi.44.070175.003453. [DOI] [PubMed] [Google Scholar]
- Felsenfeld G. Chromatin. Nature. 1978 Jan 12;271(5641):115–122. doi: 10.1038/271115a0. [DOI] [PubMed] [Google Scholar]
- Grunstein M. Hatching in the sea urchin Lytechinus pictus is accompanied by a shift in histone H4 gene activity. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4135–4139. doi: 10.1073/pnas.75.9.4135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grunstein M., Schedl P. Isolation and sequence analysis of sea urchin (Lytechinus pictus) histone H4 messenger RNA. J Mol Biol. 1976 Jun 25;104(2):323–349. doi: 10.1016/0022-2836(76)90275-8. [DOI] [PubMed] [Google Scholar]
- Hackett P. B., Traub P., Gallwitz D. The histone genes in HeLa cells are on individual transcriptional units. J Mol Biol. 1978 Dec 25;126(4):619–635. doi: 10.1016/0022-2836(78)90012-8. [DOI] [PubMed] [Google Scholar]
- Haegeman G., Fiers W. Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA. J Virol. 1978 Mar;25(3):824–830. doi: 10.1128/jvi.25.3.824-830.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johns E. W. A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7. Biochem J. 1967 Nov;105(2):611–614. doi: 10.1042/bj1050611. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KATZ A. M., DREYER W. J., ANFINSEN C. B. Peptide separation by two-dimensional chromatography and electrophoresis. J Biol Chem. 1959 Nov;234:2897–2900. [PubMed] [Google Scholar]
- Kedes L. H., Birnstiel M. L. Reiteration and clustering of DNA sequences complementary to histone messenger RNA. Nat New Biol. 1971 Apr 7;230(14):165–169. doi: 10.1038/newbio230165a0. [DOI] [PubMed] [Google Scholar]
- Kedes L. H., Chang A. C., Houseman D., Cohen S. N. Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature. 1975 Jun 12;255(5509):533–538. doi: 10.1038/255533a0. [DOI] [PubMed] [Google Scholar]
- Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
- Kedes L. H. Histone messengers and histone genes. Cell. 1976 Jul;8(3):321–331. doi: 10.1016/0092-8674(76)90144-6. [DOI] [PubMed] [Google Scholar]
- Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
- Kunkel N. S., Weinberg E. S. Histone gene transcripts in the cleavage and mesenchyme blastula embryo of the sea urchin, S. purpuratus. Cell. 1978 Jun;14(2):313–326. doi: 10.1016/0092-8674(78)90117-4. [DOI] [PubMed] [Google Scholar]
- Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
- Levis R., Penman S. 5'-terminal structures of poly(A)+ cytoplasmic messenger RNA and of poly(A)+ and poly(A)- heterogeneous nuclear RNA of cells of the dipteran Drosophila melanogaster. J Mol Biol. 1978 Apr 25;120(4):487–515. doi: 10.1016/0022-2836(78)90350-9. [DOI] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
- McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newrock K. M., Cohen L. H., Hendricks M. B., Donnelly R. J., Weinberg E. S. Stage-specific mRNAs coding for subtypes of H2A and H2B histones in the sea urchin embryo. Cell. 1978 Jun;14(2):327–336. doi: 10.1016/0092-8674(78)90118-6. [DOI] [PubMed] [Google Scholar]
- Overton G. C., Weinberg E. S. Length and sequence heterogeneity of the histone gene repeat unit of the sea urchin, S. purpuratus. Cell. 1978 Jun;14(2):247–257. doi: 10.1016/0092-8674(78)90111-3. [DOI] [PubMed] [Google Scholar]
- Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
- Pinder J. C., Staynov D. Z., Gratzer W. B. Electrophoresis of RNA in formamide. Biochemistry. 1974 Dec 17;13(26):5373–5378. doi: 10.1021/bi00723a019. [DOI] [PubMed] [Google Scholar]
- Schaffner W., Kunz G., Daetwyler H., Telford J., Smith H. O., Birnstiel M. L. Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing. Cell. 1978 Jul;14(3):655–671. doi: 10.1016/0092-8674(78)90249-0. [DOI] [PubMed] [Google Scholar]
- Stein G. S., Borun T. W. The synthesis of acidic chromosomal proteins during the cell cycle of HeLa S-3 cells. I. The accelerated accumulation of acidic residual nuclear protein before the initiation of DNA replication. J Cell Biol. 1972 Feb;52(2):292–307. doi: 10.1083/jcb.52.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stein G. S., Spelsberg T. C., Kleinsmith L. J. Nonhistone chromosomal proteins and gene regulation. Science. 1974 Mar 1;183(4127):817–824. doi: 10.1126/science.183.4127.817. [DOI] [PubMed] [Google Scholar]
- Stein G. S., Stein J. L., Thomson J. A. Chromosomal proteins in transformed and neoplastic cells: a review. Cancer Res. 1978 May;38(5):1181–1201. [PubMed] [Google Scholar]
- Stein J. L., Stein G. S., McGuire P. M. Histone messenger RNA from HeLa cells: evidence for modified 5' termini. Biochemistry. 1977 May 17;16(10):2207–2213. doi: 10.1021/bi00629a026. [DOI] [PubMed] [Google Scholar]
- Sures I., Maxam A., Cohn R. H., Kedes L. H. Identification and location of the histone H2A and H3 genes by sequence analysis of sea urchin (S. purpuratus) DNA cloned in E. coli. Cell. 1976 Dec;9(4 Pt 1):495–502. doi: 10.1016/0092-8674(76)90031-3. [DOI] [PubMed] [Google Scholar]
- Thrall C. L., Lichtler A., Stein J. L., Stein G. S. In vitro synthesis of single-stranded DNA complementary to histone messenger RNAs. Methods Cell Biol. 1978;19:237–255. doi: 10.1016/s0091-679x(08)60027-8. [DOI] [PubMed] [Google Scholar]
- Weinberg E. S., Birnstiel M. L., Purdom I. F., Williamson R. Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence. Nature. 1972 Nov 24;240(5378):225–228. doi: 10.1038/240225a0. [DOI] [PubMed] [Google Scholar]
- Wilson M. C., Melli M. Determination of the number of histone genes in human DNA. J Mol Biol. 1977 Mar 5;110(3):511–535. doi: 10.1016/s0022-2836(77)80110-1. [DOI] [PubMed] [Google Scholar]