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. 1980 Apr;77(4):1947–1950. doi: 10.1073/pnas.77.4.1947

Sendai virus receptor: proposed recognition structure based on binding to plastic-adsorbed gangliosides.

J Holmgren, L Svennerholm, H Elwing, P Fredman, O Strannegård
PMCID: PMC348626  PMID: 6246515

Abstract

The binding of Sendai virus to polystyrene petri dishes to which various gangliosides of defined structures had been adsorbed was determined. The ganglioside-bound virus was visualized either by a water vapor condensation method or by a hemadsorption method. By either assay, specific virus binding of high affinity was demonstrated to the gangliosides GT1a, GQ1b, and GPlc which have a common end sequence in the oligosaccharide moiety: NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3Gal beta 1 leads to 3GalNAc leads to. Binding also occurred to the GD1a and GT1b gangliosides, which have the same end carbohydrate sequence except for the terminal N-acetylneuraminic acid, but the affinity was only 1-9% of that of the gangliosides with a terminal disialosyl linkage. It is proposed that the structure NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3Gal beta 1 leads to 3GalNAc is the recognition-specific structure of the receptor for Sendai virus that is present on cell membrane gangliosides and possibly also glycoproteins. Binding tests to plastic-adsorbed glycolipids are suggested to be a useful tool for identification of the receptor recognition structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Elwing H., Nilsson L. A., Ouchterlony O. A simple spot technique for thin layer immunoassays (TIA) on plastic surfaces. J Immunol Methods. 1977;17(1-2):131–145. doi: 10.1016/0022-1759(77)90084-9. [DOI] [PubMed] [Google Scholar]
  2. Elwing H., Nilsson L., Ouchterlony O. Visualization principles in thin-layer immunoassays (TIA) on plastic surfaces. Int Arch Allergy Appl Immunol. 1976;51(6):757–762. doi: 10.1159/000231654. [DOI] [PubMed] [Google Scholar]
  3. Haywood A. M. Characteristics of Sendai virus receptors in a model membrane. J Mol Biol. 1974 Mar 15;83(4):427–436. doi: 10.1016/0022-2836(74)90504-x. [DOI] [PubMed] [Google Scholar]
  4. Holmgren J., Lönnroth I., Svennerholm L. Tissue receptor for cholera exotoxin: postulated structure from studies with GM1 ganglioside and related glycolipids. Infect Immun. 1973 Aug;8(2):208–214. doi: 10.1128/iai.8.2.208-214.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howe C., Lee L. T. Virus-erythrocyte interactions. Adv Virus Res. 1972;17:1–50. doi: 10.1016/S0065-3527(08)60746-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. King C. A., Van Heyningen W. E. Deactivation of cholera toxin by a sialidase-resistant monosialosylganglioside. J Infect Dis. 1973 Jun;127(6):639–647. doi: 10.1093/infdis/127.6.639. [DOI] [PubMed] [Google Scholar]
  7. Mansson J. E., Vanier M. T., Svennerholm L. Changes in the fatty acid and sphingosine composition of the major gangliosides of human brain with age. J Neurochem. 1978 Jan;30(1):273–275. doi: 10.1111/j.1471-4159.1978.tb07064.x. [DOI] [PubMed] [Google Scholar]
  8. Mullin B. R., Fishman P. H., Lee G., Aloj S. M., Ledley F. D., Winand R. J., Kohn L. D., Brady R. O. Thyrotropin-ganglioside interactions and their relationship to the structure and function of thyrotropin receptors. Proc Natl Acad Sci U S A. 1976 Mar;73(3):842–846. doi: 10.1073/pnas.73.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Paulson J. C., Sadler J. E., Hill R. L. Restoration of specific myxovirus receptors to asialoerythrocytes by incorporation of sialic acid with pure sialyltransferases. J Biol Chem. 1979 Mar 25;254(6):2120–2124. [PubMed] [Google Scholar]
  10. Rearick J. I., Sadler J. E., Paulson J. C., Hill R. L. Enzymatic characterization of beta D-galactoside alpha2 leads to 3 sialyltransferase from porcine submaxillary gland. J Biol Chem. 1979 Jun 10;254(11):4444–4451. [PubMed] [Google Scholar]
  11. Svennerholm L., Fredman P. A procedure for the quantitative isolation of brain gangliosides. Biochim Biophys Acta. 1980 Jan 18;617(1):97–109. doi: 10.1016/0005-2760(80)90227-1. [DOI] [PubMed] [Google Scholar]
  12. Svennerholm L., Vanier M. T., Månsson J. E. Krabbe disease: a galactosylsphingosine (psychosine) lipidosis. J Lipid Res. 1980 Jan;21(1):53–64. [PubMed] [Google Scholar]
  13. Vengris V. E., Reynolds F. H., Jr, Hollenberg M. D., Pitha P. M. Interferon action: role of membrane gangliosides. Virology. 1976 Jul 15;72(2):486–493. doi: 10.1016/0042-6822(76)90177-x. [DOI] [PubMed] [Google Scholar]

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