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. 1980 Apr;77(4):2153–2157. doi: 10.1073/pnas.77.4.2153

Evidence of RNA in D loops of intracellular lambda DNA.

D K Chattoraj, F W Stahl
PMCID: PMC348670  PMID: 6445565

Abstract

If lambda DNA replication is blocked by mutation in any one of several genes essential for replication, intracellular lambda DNA often shows short three-stranded regions called D loops. In this report we show that one arm of a D loop is an RNA . DNA hybrid, whereas the remaining arm is made up of single-stranded DNA. The RNA can be partially removed by RNase A and totally removed by RNase H. Also, D loops do not appear if infections are made in cells treated with rifampin, a potent inhibitor of transcription by Escherichia coli RNA polymerase. Several genes associated with recombination, including the host recA gene, are not essential for D-loop formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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