Scheme 3.
Reactivity of GFP(S147C) sulfonium 8 (100 μL, 0.9 mg mL−1 in H2O/DMF 10:1, pH 8, 100 mM phosphate) with β-mercaptoethanol (5 μL, 680 mM in H2O). a) HSCH2CH2OH (1000 equiv), 37 °C, 2.5 h, DMF, H2O, pH 8 (phosphate), >95 % conversion.
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