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. 1981 Sep;78(9):5315–5319. doi: 10.1073/pnas.78.9.5315

Localization and nucleotide sequence of the gene coding for the woodchuck hepatitis virus surface antigen: comparison with the gene coding for the human hepatitis B virus surface antigen.

F Galibert, T N Chen, E Mandart
PMCID: PMC348735  PMID: 6946471

Abstract

Nucleotide sequence analysis was performed with the Maxam--Gilbert method on a cloned woodchuck hepatitis virus DNA (Eco WHV DNA). The structural gene coding for the envelope protein of the virus was localized on the viral genome in the partially single-stranded region between map positions 91.2 and 71. This gene is composed of 669 nucleotides and can code for a polypeptide of 25,645 daltons. The DNA sequence and the deduced amino acid sequence were compared with those of the corresponding gene and surface antigen of the related hepatitis B virus, allowing some insight into the localization of the antigenic site.

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Selected References

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  1. Breathnach R., Benoist C., O'Hare K., Gannon F., Chambon P. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853–4857. doi: 10.1073/pnas.75.10.4853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burrell C. J., Mackay P., Greenaway P. J., Hofschneider P. H., Murray K. Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322. Nature. 1979 May 3;279(5708):43–47. doi: 10.1038/279043a0. [DOI] [PubMed] [Google Scholar]
  3. Catterall J. F., O'Malley B. W., Robertson M. A., Staden R., Tanaka Y., Brownlee G. G. Nucleotide sequence homology at 12 intron--exon junctions in the chick ovalbumin gene. Nature. 1978 Oct 12;275(5680):510–513. doi: 10.1038/275510a0. [DOI] [PubMed] [Google Scholar]
  4. Charnay P., Mandart E., Hampe A., Fitoussi F., Tiollais P., Galibert F. Localization on the viral genome and nucleotide sequence of the gene coding for the two major polypeptides of the hepatitis B surface antigen (HBs Ag). Nucleic Acids Res. 1979 Sep 25;7(2):335–346. doi: 10.1093/nar/7.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cummings I. W., Browne J. K., Salser W. A., Tyler G. V., Snyder R. L., Smolec J. M., Summers J. Isolation, characterization, and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1842–1846. doi: 10.1073/pnas.77.4.1842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fritsch A., Pourcel C., Charnay P., Tiollais P. Clonage du génome du virus de l'hépatite B dans Escherichia coli. C R Acad Sci Hebd Seances Acad Sci D. 1978 Dec;287(16):1453–1456. [PubMed] [Google Scholar]
  7. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charnay P. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979 Oct 25;281(5733):646–650. doi: 10.1038/281646a0. [DOI] [PubMed] [Google Scholar]
  8. Hartley J. L., Donelson J. E. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860–865. doi: 10.1038/286860a0. [DOI] [PubMed] [Google Scholar]
  9. Hérissé J., Courtois G., Galibert F. Nucleotide sequence of the EcoRI D fragment of adenovirus 2 genome. Nucleic Acids Res. 1980 May 24;8(10):2173–2192. doi: 10.1093/nar/8.10.2173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. DNA polymerase associated with human hepatitis B antigen. J Virol. 1973 Nov;12(5):995–1005. doi: 10.1128/jvi.12.5.995-1005.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  13. Ohori H., Onodera S., Ishida N. Demonstration of hepatitis B e antigen (HBeAg) in association with intact Dane particles. J Gen Virol. 1979 May;43(2):423–427. doi: 10.1099/0022-1317-43-2-423. [DOI] [PubMed] [Google Scholar]
  14. Pasek M., Goto T., Gilbert W., Zink B., Schaller H., MacKay P., Leadbetter G., Murray K. Hepatitis B virus genes and their expression in E. coli. Nature. 1979 Dec 6;282(5739):575–579. doi: 10.1038/282575a0. [DOI] [PubMed] [Google Scholar]
  15. Rao K. R., Vyas G. N. Biochemical characterization of hepatitis B surface antigen in relation to serologic activity. J Biol Stand. 1976;4(4):295–304. doi: 10.1016/s0092-1157(76)80014-5. [DOI] [PubMed] [Google Scholar]
  16. Sninsky J. J., Siddiqui A., Robinson W. S., Cohen S. N. Cloning and endonuclease mapping of the hepatitis B viral genome. Nature. 1979 May 24;279(5711):346–348. doi: 10.1038/279346a0. [DOI] [PubMed] [Google Scholar]
  17. Staden R. Sequence data handling by computer. Nucleic Acids Res. 1977 Nov;4(11):4037–4051. doi: 10.1093/nar/4.11.4037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Struck D. K., Lennarz W. J., Brew K. Primary structural requirements for the enzymatic formation of the N-glycosidic bond in glycoproteins. Studies with alpha-lactalbumin. J Biol Chem. 1978 Aug 25;253(16):5786–5794. [PubMed] [Google Scholar]
  19. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H. M., Rutter W. J. Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature. 1979 Aug 30;280(5725):815–819. doi: 10.1038/280815a0. [DOI] [PubMed] [Google Scholar]
  21. Werner B. G., O'Connell A. P., Summers J. Association of e antigen with Dane particle DNA in sera from asymptomatic carriers of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977 May;74(5):2149–2151. doi: 10.1073/pnas.74.5.2149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Werner B. G., Smolec J. M., Snyder R., Summers J. Serological relationship of woodchuck hepatitis virus to human hepatitis B virus. J Virol. 1979 Oct;32(1):314–322. doi: 10.1128/jvi.32.1.314-322.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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