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. 1981 Sep;78(9):5411–5415. doi: 10.1073/pnas.78.9.5411

Identification of a RNA polymerase II initiation site in the long terminal repeat of Moloney murine leukemia viral DNA.

S A Fuhrman, C Van Beveren, I M Verma
PMCID: PMC348755  PMID: 6946480

Abstract

We have used a soluble in vitro RNA polymerase II transcription system to define the site of initiation of Moloney murine leukemia viral RNA synthesis. Molecularly cloned integrated and unintegrated Moloney murine leukemia virus DNAs were used as templates. The 5' ends of in vitro transcripts and virion RNA of Moloney murine leukemia virus were compared by nuclease S1 protection experiments. Our results indicate that viral sequences upstream of the in vivo cap site are implicated in the transcription of viral RNA and that the 5' end of an in vitro transcript derived from an integrated Moloney murine leukemia virus clone corresponds to the 5' end of viral genomic RNA.

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Selected References

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