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. 1981 Sep;78(9):5724–5728. doi: 10.1073/pnas.78.9.5724

DNA-mediated gene transfer of beta-aspartylhydroxamate resistance into Chinese hamster ovary cells.

I L Andrulis, L Siminovitch
PMCID: PMC348842  PMID: 6117859

Abstract

Cell lines that have high levels of resistance to beta-aspartylhydroxamate and elevated levels of asparagine synthetase activity were selected in two steps from Chinese hamster ovary cells. Resistance to beta-aspartylhydroxmate was transferred into sensitive cells by using total genomic DNA derived from the dominant two-step mutants. The surviving colonies were characterized as transferants on the basis of transfer frequency, degree of resistance to beta-aspartylhydroxamate, increased level of asparagine synthetase activity, expression of the donor form of asparagine synthetase, codominance in hybrids, and instability of the phenotype in the absence of selection.

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Selected References

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