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. 1981 Oct;78(10):5983–5987. doi: 10.1073/pnas.78.10.5983

Molecular cloning of the genome of poliovirus type 1.

S van der Werf, F Bregegere, H Kopecka, N Kitamura, P G Rothberg, P Kourilsky, E Wimmer, M Girard
PMCID: PMC348961  PMID: 6273836

Abstract

Poliovirus cDNA.RNA hybrids were prepared from the Mahoney strain of poliovirus type 1 by using reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) and cloned in the Escherichia coli plasmid pBR322. Bacteria colonies carrying recombinant plasmids were selected by in situ hybridization with virus-specific RNase T1-resistant oligonucleotides. Analysis of the cDNA inserts by restriction mapping and electron microscopy showed that the cloned cDNAs, the longest of which was 3.2 kilobase pairs, originated from various parts of the viral RNA, covering at least 99% of the genome length. Due to overlapping of the clones, the restriction map of the poliovirus genome could be reconstructed. The complete 5' end of the genome was successfully cloned in at least one of the recombinant plasmids, pPV1-366.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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