Abstract
5'-O-Monophosphoryladenylyl(2' goes to 5')adenylyl-(2' goes to 5')adenosine [(2' goes to 5')(pA)3] antagonizes the protein synthesis inhibitory effects of 5'-O-triphosphoryladenylyl-(2' goes to 5')adenylyl(2' goes to 5')adenosine by preventing activation of the (2' goes to 5')oligo(a)-activated ribonuclease which degrades mRNA. The oligoribonucleotide (2' goes to 5')(pA)3 also antagonizes the translational inhibitory capacity of poly(I).poly(C) in extracts of interferon-treated L cells, suggesting that (2' goes to 5')oligo(A) is the primary mediator of the protein synthesis inhibitory effects of poly(I).poly(C) in this cell-free system.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baglioni C. Interferon-induced enzymatic activities and their role in the antriviral state. Cell. 1979 Jun;17(2):255–264. doi: 10.1016/0092-8674(79)90151-x. [DOI] [PubMed] [Google Scholar]
- Baglioni C., Minks M. A., Maroney P. A. Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A. Nature. 1978 Jun 22;273(5664):684–687. doi: 10.1038/273684a0. [DOI] [PubMed] [Google Scholar]
- Ball L. A., White C. N. Nuclease activation by double-stranded RNA and by 2',5'-oligoadenylate in extracts of interferon-treated chick cells. Virology. 1979 Mar;93(2):348–356. doi: 10.1016/0042-6822(79)90239-3. [DOI] [PubMed] [Google Scholar]
- Chernajovsky Y., Kimchi A., Schmidt A., Zilberstein A., Revel M. Differential effects of two interferon-induced translational inhibitors on initiation of protein synthesis. Eur J Biochem. 1979 May 2;96(1):35–41. doi: 10.1111/j.1432-1033.1979.tb13010.x. [DOI] [PubMed] [Google Scholar]
- Clemens M. J., Williams B. R. Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts. Cell. 1978 Mar;13(3):565–572. doi: 10.1016/0092-8674(78)90329-x. [DOI] [PubMed] [Google Scholar]
- Dougherty J. P., Samanta H., Farrell P. J., Lengyel P. Interferon, double-stranded RNA, and RNA degradation. Isolation of homogeneous pppA(2'p5'A)n-1 synthetase from Ehrlich ascites tumor cells. J Biol Chem. 1980 May 10;255(9):3813–3816. [PubMed] [Google Scholar]
- Eppstein D. A., Samuel C. E. Mechanism of interferon action. Properties of an interferon-mediated ribonucleolytic activity from mouse L929 cells. Virology. 1978 Aug;89(1):240–251. doi: 10.1016/0042-6822(78)90056-9. [DOI] [PubMed] [Google Scholar]
- Epstein D. A., Torrence P. F., Friedman R. M. Double-stranded RNA inhibits a phosphoprotein phosphatase present in interferon-treated cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):107–111. doi: 10.1073/pnas.77.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ernst V., Levin D. H., Leroux A., London I. M. Site-specific phosphorylation of the alpha subunit of eukaryotic initiation factor eIF-2 by the heme-regulated and double-stranded RNA-activated eIF-2 alpha kinases from rabbit reticulocyte lysates. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1286–1290. doi: 10.1073/pnas.77.3.1286. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farrell P. J., Sen G. C., Dubois M. F., Ratner L., Slattery E., Lengyel P. Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5893–5897. doi: 10.1073/pnas.75.12.5893. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hovanessian A. G., Brown R. E., Kerr I. M. Synthesis of low molecular weight inhibitor of protein synthesis with enzyme from interferon-treated cells. Nature. 1977 Aug 11;268(5620):537–540. doi: 10.1038/268537a0. [DOI] [PubMed] [Google Scholar]
- Hovanessian A. G. Intracellular events in interferon-treated cells. Differentiation. 1979;15(3):139–151. doi: 10.1111/j.1432-0436.1979.tb01044.x. [DOI] [PubMed] [Google Scholar]
- Jagus R., Anderson W. F., Safer B. The regulation of initiation of mammalian protein synthesis. Prog Nucleic Acid Res Mol Biol. 1981;25:127–185. doi: 10.1016/s0079-6603(08)60484-5. [DOI] [PubMed] [Google Scholar]
- Johnston M. I., Friedman R. M., Torrence P. F. Interferon-induced (2'-5')oligoadenylate synthetase: adsorpton to and assay on adenosine 2',5'-diphosphate-sepharose. Biochemistry. 1980 Nov 25;19(24):5580–5585. doi: 10.1021/bi00565a019. [DOI] [PubMed] [Google Scholar]
- Johnston M. I., Zoon K. C., Friedman R. M., De Clercq E., Torrence P. F. Oligo(2'-5')adenylate synthetase in human lymphoblastoid cells. Biochem Biophys Res Commun. 1980 Nov 28;97(2):375–383. doi: 10.1016/0006-291x(80)90275-2. [DOI] [PubMed] [Google Scholar]
- Kerr I. M., Brown R. E., Ball L. A. Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatment. Nature. 1974 Jul 5;250(461):57–59. doi: 10.1038/250057a0. [DOI] [PubMed] [Google Scholar]
- Kerr I. M., Brown R. E. pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells. Proc Natl Acad Sci U S A. 1978 Jan;75(1):256–260. doi: 10.1073/pnas.75.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kimchi A., Shure H., Revel M. Regulation of lymphocyte mitogenesis by (2'--5') oligo-isoadenylate. Nature. 1979 Dec 20;282(5741):849–851. doi: 10.1038/282849a0. [DOI] [PubMed] [Google Scholar]
- Knight M., Cayley P. J., Silverman R. H., Wreschner D. H., Gilbert C. S., Brown R. E., Kerr I. M. Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells. Nature. 1980 Nov 13;288(5787):189–192. doi: 10.1038/288189a0. [DOI] [PubMed] [Google Scholar]
- Krishnan I., Baglioni C. 2'5' oligo(A) polymerase activity in serum of mice infected with EMC virus or treated with interferon. Nature. 1980 Jun 12;285(5765):485–488. doi: 10.1038/285485a0. [DOI] [PubMed] [Google Scholar]
- Krishnan I., Baglioni C. Increased levels of (2'-5')oligo(A) polymerase activity in human lymphoblastoid cells treated with glucocorticoids. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6506–6510. doi: 10.1073/pnas.77.11.6506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lebleu B., Sen G. C., Shaila S., Cabrer B., Lengyel P. Interferon, double-stranded RNA, and protein phosphorylation. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3107–3111. doi: 10.1073/pnas.73.9.3107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin E. M., Birdsall N. J., Brown R. E., Kerr I. M. Enzymic synthesis, characterisation and nuclear-magnetic-resonance spectra of pppA2'p5'A2'p5'A and related oligonucleotides: comparison with chemically synthesised material. Eur J Biochem. 1979 Apr 2;95(2):295–307. doi: 10.1111/j.1432-1033.1979.tb12965.x. [DOI] [PubMed] [Google Scholar]
- Roberts W. K., Clemens M. J., Kerr I. M. Interferon-induced inhibition of protein synthesis in L-cell extracts: an ATP-dependent step in the activation of an inhibitor by double-stranded RNA. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3136–3140. doi: 10.1073/pnas.73.9.3136. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Samanta H., Dougherty J. P., Lengyel P. Synthesis of (2'-5')(A)n from ATP. Characteristics of the reaction catalyzed by (2'-5')(A)n synthetase purified from mouse Ehrlich ascites tumor cells treated with interferon. J Biol Chem. 1980 Oct 25;255(20):9807–9813. [PubMed] [Google Scholar]
- Samuel C. E. Mechanism of interferon action. Kinetics of interferon action in mouse L929 cells: phosphorylation of protein synthesis initiation factor elF-2 and ribosome-associated protein P1. Virology. 1979 Feb;93(1):281–285. doi: 10.1016/0042-6822(79)90300-3. [DOI] [PubMed] [Google Scholar]
- Schmidt A., Chernajovsky Y., Shulman L., Federman P., Berissi H., Revel M. An interferon-induced phosphodiesterase degrading (2'-5') oligoisoadenylate and the C-C-A terminus of tRNA. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4788–4792. doi: 10.1073/pnas.76.10.4788. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimizu N., Sokawa Y. 2',5'-Oligoadenylate synthetase activity in lymphocytes from normal mouse. J Biol Chem. 1979 Dec 10;254(23):12034–12037. [PubMed] [Google Scholar]
- Slattery E., Ghosh N., Samanta H., Lengyel P. Interferon, double-stranded RNA, and RNA degradation: activation of an endonuclease by (2'-5')An. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4778–4782. doi: 10.1073/pnas.76.10.4778. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sokawa Y., Ando T., Ishihara Y. Induction of 2',5'-oligoadenylate synthetase and interferon in mouse trigeminal ganglia infected with herpes simplex virus. Infect Immun. 1980 Jun;28(3):719–723. doi: 10.1128/iai.28.3.719-723.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stark G. R., Dower W. J., Schimke R. T., Brown R. E., Kerr I. M. 2-5A synthetase: assay, distribution and variation with growth or hormone status. Nature. 1979 Mar 29;278(5703):471–473. doi: 10.1038/278471a0. [DOI] [PubMed] [Google Scholar]
- Torrence P. F., Friedman R. M. Are double-stranded RNA-directed inhibition of protein synthesis in interferon-treated cells and interferon induction related phenomena? J Biol Chem. 1979 Feb 25;254(4):1259–1267. [PubMed] [Google Scholar]
- Vandenbussche P., Content J., Lebleu B., Werenne J. Comparison of interferon action in interferon resistant and sensitive L1210 cells. J Gen Virol. 1978 Oct;41(1):161–166. doi: 10.1099/0022-1317-41-1-161. [DOI] [PubMed] [Google Scholar]
- Wallach D., Revel M. An interferon-induced cellular enzyme is incorporated into virions. Nature. 1980 Sep 4;287(5777):68–70. doi: 10.1038/287068a0. [DOI] [PubMed] [Google Scholar]
- Williams B. R., Golgher R. R., Brown R. E., Gilbert C. S., Kerr I. M. Natural occurrence of 2-5A in interferon-treated EMC virus-infected L cells. Nature. 1979 Dec 6;282(5739):582–586. doi: 10.1038/282582a0. [DOI] [PubMed] [Google Scholar]
- Williams B. R., Kerr I. M., Gilbert C. S., White C. N., Ball L. A. Synthesis and breakdown of pppA2'p5'A2'p5'A and transient inhibiton of protein synthesis in extracts from interferon-treated and control cells. Eur J Biochem. 1978 Dec;92(2):455–462. doi: 10.1111/j.1432-1033.1978.tb12767.x. [DOI] [PubMed] [Google Scholar]
- Wreschner D. H., McCauley J. W., Skehel J. J., Kerr I. M. Interferon action--sequence specificity of the ppp(A2'p)nA-dependent ribonuclease. Nature. 1981 Jan 29;289(5796):414–417. doi: 10.1038/289414a0. [DOI] [PubMed] [Google Scholar]
- Zilberstein A., Federman P., Shulman L., Revel M. Specific phosphorylation in vitro of a protein associated with ribosomes of interferon-treated mouse L cells. FEBS Lett. 1976 Sep 15;68(1):119–124. doi: 10.1016/0014-5793(76)80418-8. [DOI] [PubMed] [Google Scholar]