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. 1981 Oct;78(10):6513–6516. doi: 10.1073/pnas.78.10.6513

Amygdalin (Laetrile) and prunasin beta-glucosidases: distribution in germ-free rat and in human tumor tissue.

J Newmark, R O Brady, P M Grimley, A E Gal, S G Waller, J R Thistlethwaite
PMCID: PMC349070  PMID: 6796962

Abstract

Amygdalin, the gentiobioside derivative of mandelonitrile commonly referred to as Laetrile, is presently under intensive investigation as a potential cancer chemotherapeutic agent. Because of this interest, we investigated the activity of beta-glucosidases that cleave glucose from amygdalin and from prunasin (mandelonitrile monoglucoside) in tissues from germ-free rats and in normal and neoplastic human tissues. Rat and human small intestinal mucosa contain high levels of activity of glucosidases that act on both of these cyanogenic glucosides. Release of glucose from these compounds was not detected in any of the human neoplastic tissues examined in the present study. These observations are consistent with reports of cyanide toxicity through the oral use of amygdalin or prunasin and pose serious questions concerning the alleged tumoricidal effect of amygdalin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames M. M., Kovach J. S., Flora K. P. Initial pharmacologic studies of amygdalin (laetrile) in man. Res Commun Chem Pathol Pharmacol. 1978 Oct;22(1):175–185. [PubMed] [Google Scholar]
  2. Brady R. O., Gal A. E., Kanfer J. N., Bradley R. M. The metabolism of glucocerebrosides. 3. Purification and properties of a glucosyl- and galactosylceramide-cleaving enzyme from rat intestinal tissue. J Biol Chem. 1965 Oct;240(10):3766–3770. [PubMed] [Google Scholar]
  3. Davignon J. P., Trissel L. A., Kleinman L. M. Pharmaceutical assessment of amygdalin (Laetrile) products. Cancer Treat Rep. 1978 Jan;62(1):99–104. [PubMed] [Google Scholar]
  4. Ellison N. M., Byar D. P., Newell G. R. Special report on Laetrile: the NCI Laetrile Review. Results of the National Cancer Institute's retrospective Laetrile analysis. N Engl J Med. 1978 Sep 7;299(10):549–552. doi: 10.1056/NEJM197809072991013. [DOI] [PubMed] [Google Scholar]
  5. Freese A., Brady R. O., Gal A. E. A beta-glucosidase in feline kidney that hydrolyzes amygdalin (laetrile). Arch Biochem Biophys. 1980 May;201(2):363–368. doi: 10.1016/0003-9861(80)90523-8. [DOI] [PubMed] [Google Scholar]
  6. Furbish F. S., Blair H. E., Shiloach J., Pentchev P. G., Brady R. O. Enzyme replacement therapy in Gaucher's disease: large-scale purification of glucocerebrosidase suitable for human administration. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3560–3563. doi: 10.1073/pnas.74.8.3560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ginns E. I., Brady R. O., Stowens D. W., Furbish F. S., Barranger J. A. A new group of glucocerebrosidase isozymes found in human white blood cells. Biochem Biophys Res Commun. 1980 Dec 16;97(3):1103–1107. doi: 10.1016/0006-291x(80)91489-8. [DOI] [PubMed] [Google Scholar]
  8. Haisman D. R., Knight D. J. The enzymic hydrolysis of amygdalin. Biochem J. 1967 May;103(2):528–534. doi: 10.1042/bj1030528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill G. J., 2nd, Shine T. E., Hill H. Z., Miller C. Failure of amygdalin to arrest B16 melanoma and BW5147 AKR leukemia. Cancer Res. 1976 Jun;36(6):2102–2107. [PubMed] [Google Scholar]
  10. Holden C. Laetrile: "quack" cancer remedy still brings hope to sufferers. Science. 1976 Sep 10;193(4257):982–985. doi: 10.1126/science.193.4257.982. [DOI] [PubMed] [Google Scholar]
  11. Khandekar J. D., Edelman H. Studies of amygdalin (laetrile) toxicity in rodents. JAMA. 1979 Jul 13;242(2):169–171. [PubMed] [Google Scholar]
  12. Kobayashi T., Suzuki K. The glycosylceramidase in the murine intestine. Purification and substrate specificity. J Biol Chem. 1981 Aug 10;256(15):7768–7773. [PubMed] [Google Scholar]
  13. LEVI L., FRENCH W. N., BICKIS I. J., HENDERSON I. W. LAETRILE: A STUDY OF ITS PHYSICOCHEMICAL AND BIOCHEMICAL PROPERTIES. Can Med Assoc J. 1965 May 15;92:1057–1061. [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laster W. R., Jr, Schabel F. M., Jr Experimental studies of the antitumor activity of amygdalin MF (NSC-15780) alone and in combination with beta-glucosidase (NSC-128056). Cancer Chemother Rep. 1975 Sep-Oct;59(5):951–965. [PubMed] [Google Scholar]
  16. Moertel C. G., Ames M. M., Kovach J. S., Moyer T. P., Rubin J. R., Tinker J. H. A pharmacologic and toxicological study of amygdalin. JAMA. 1981 Feb 13;245(6):591–594. [PubMed] [Google Scholar]
  17. Schmidt E. S., Newton G. W., Sanders S. M., Lewis J. P., Conn E. E. Laetrile toxicity studies in dogs. JAMA. 1978 Mar 6;239(10):943–947. [PubMed] [Google Scholar]
  18. Shibata S., Nakanishi H. Structure of the sugar moiety in the nephritogenic glycopeptide from rat glomerular, basement membrane. Carbohydr Res. 1980 Mar 15;81(2):345–348. doi: 10.1016/s0008-6215(00)85667-0. [DOI] [PubMed] [Google Scholar]
  19. Shibata S., Nakanishi H. The alpha-D configuration of the glycosyl-N linkage in the nephritogenic glycopeptide isolated from rat glomerular, basement membrane. Carbohydr Res. 1980 Nov 15;86(2):316–320. doi: 10.1016/s0008-6215(00)85911-x. [DOI] [PubMed] [Google Scholar]
  20. Sun M. Laetrile brush fire is out, scientists hope. Science. 1981 May 15;212(4496):758–759. doi: 10.1126/science.7221562. [DOI] [PubMed] [Google Scholar]
  21. Tamura G., Gold C., Ferro-Luzzi A., Ames B. N. Fecalase: a model for activation of dietary glycosides to mutagens by intestinal flora. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4961–4965. doi: 10.1073/pnas.77.8.4961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wodinsky I., Swiniarski J. K. Antitumor activity of amygdalin MF (NSC-15780) as a single agent and with beta-glucosidase (NSC-128056) on a spectrum of transplantable rodent tumors. Cancer Chemother Rep. 1975 Sep-Oct;59(5):939–950. [PubMed] [Google Scholar]

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