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. 1981 Oct;78(10):6531–6534. doi: 10.1073/pnas.78.10.6531

Dopaminergic modulation of adenylate cyclase stimulation by vasoactive intestinal peptide in anterior pituitary.

P Onali, J P Schwartz, E Costa
PMCID: PMC349074  PMID: 6171819

Abstract

The activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] by vasoactive intestinal peptide (VIP) was used as a model to investigate the molecular mechanisms triggered by the occupancy of dopamine recognition sites in rat anterior pituitary. Dopamine failed to change the basal enzyme activity, but it inhibited the stimulation of adenylate cyclase elicited by VIP. Apomorphine, 2-amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene, and 2-bromo-alpha-ergocryptine mimicked the effect of dopamine, whereas (-)-sulpiride and and classical neuroleptics antagonized it. Dopamine failed to modulate the activation of pituitary adenylate cyclase by prostaglandin E1, which does not increase prolactin secretion. From these results we infer that stimulation of D-2 (dopamine) receptors may affect pituitary secretion by inhibiting the activation of anterior pituitary adenylate cyclase by VIP or other secretagogues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahn H. S., Gardner E., Makman M. H. Anterior pituitary adenylate cyclase: stimulation by dopamine and other monoamines. Eur J Pharmacol. 1979 Jan 15;53(3):313–317. doi: 10.1016/0014-2999(79)90139-0. [DOI] [PubMed] [Google Scholar]
  2. Besser G. M., Parke L., Edwards C. R., Forsyth I. A., McNeilly A. S. Galactorrhoea: successful treatment with reduction of plasma prolactin levels by brom-ergocryptine. Br Med J. 1972 Sep 16;3(5828):669–672. doi: 10.1136/bmj.3.5828.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borghi C., Nicosia S., Giachetti A., Said S. I. Adenylate cyclase of rat pituitary gland: stimulation by vasoactive intestinal polypeptide (VIP). FEBS Lett. 1979 Dec 15;108(2):403–406. doi: 10.1016/0014-5793(79)80573-6. [DOI] [PubMed] [Google Scholar]
  4. Böhlen P., Stein S., Dairman W., Udenfriend S. Fluorometric assay of proteins in the nanogram range. Arch Biochem Biophys. 1973 Mar;155(1):213–220. doi: 10.1016/s0003-9861(73)80023-2. [DOI] [PubMed] [Google Scholar]
  5. Calabro M. A., MacLeod R. M. Binding of dopamine to bovine anterior pituitary gland membranes. Neuroendocrinology. 1978;25(1):32–46. doi: 10.1159/000122762. [DOI] [PubMed] [Google Scholar]
  6. Caron M. G., Beaulieu M., Raymond V., Gagné B., Drouin J., Lefkowitz R. J., Labrie F. Dopaminergic receptors in the anterior pituitary gland. Correlation of [3H]dihydroergocryptine binding with the dopaminergic control of prolactin release. J Biol Chem. 1978 Apr 10;253(7):2244–2253. [PubMed] [Google Scholar]
  7. Clemens J. A., Shaar C. J., Smalstig E. B., Bach N. J., Kornfeld E. C. Inhibition of prolactin secretion by ergolines. Endocrinology. 1974 Apr;94(4):1171–1176. doi: 10.1210/endo-94-4-1171. [DOI] [PubMed] [Google Scholar]
  8. Cote T. E., Grewe C. W., Kebabian J. W. Stimulation of a D-2 dopamine receptor in the intermediate lobe of the rat pituitary gland decreases the responsiveness of the beta-adrenoceptor: biochemical mechanism. Endocrinology. 1981 Feb;108(2):420–426. doi: 10.1210/endo-108-2-420. [DOI] [PubMed] [Google Scholar]
  9. Cronin M. J., Roberts J. M., Weiner R. I. Dopamine and dihydroergocryptine binding to the anterior pituitary and other brain areas of the rat and sheep. Endocrinology. 1978 Jul;103(1):302–309. doi: 10.1210/endo-103-1-302. [DOI] [PubMed] [Google Scholar]
  10. De Camilli P., Macconi D., Spada A. Dopamine inhibits adenylate cyclase in human prolactin-secreting pituitary adenomas. Nature. 1979 Mar 15;278(5701):252–254. doi: 10.1038/278252a0. [DOI] [PubMed] [Google Scholar]
  11. Drouin J., Labrie F. Specificity of the stimulatory effect of prostaglandins on hormone release in rat anterior pituitary cells in culture. Prostaglandins. 1976 Feb;11(2):355–365. doi: 10.1016/0090-6980(76)90157-x. [DOI] [PubMed] [Google Scholar]
  12. Enjalbert A., Arancibia S., Ruberg M., Priam M., Bluet-Pajot M. T., Rotsztejn W. H., Kordon C. Stimulation of in vitro prolactin release by vasoactive intestinal peptide. Neuroendocrinology. 1980 Sep;31(3):200–204. doi: 10.1159/000123074. [DOI] [PubMed] [Google Scholar]
  13. Gräf K. J., Neumann F., Horowski R. Effect of the ergot derivative lisuride hydrogen maleate on serum prolactin concentrations in female rats. Endocrinology. 1976 Mar;98(3):598–605. doi: 10.1210/endo-98-3-598. [DOI] [PubMed] [Google Scholar]
  14. Kato Y., Iwasaki Y., Iwasaki J., Abe H., Yanaihara N., Imura H. Prolactin release by vasoactive intestinal polypeptide in rats. Endocrinology. 1978 Aug;103(2):554–558. doi: 10.1210/endo-103-2-554. [DOI] [PubMed] [Google Scholar]
  15. Kebabian J. W., Calne D. B., Kebabian P. R. Lergotrile mesylate: an in vivo dopamine agonist which blocks dopamine receptors in vitro. Commun Psychopharmacol. 1977;1(4):311–318. [PubMed] [Google Scholar]
  16. Kebabian J. W., Calne D. B. Multiple receptors for dopamine. Nature. 1979 Jan 11;277(5692):93–96. doi: 10.1038/277093a0. [DOI] [PubMed] [Google Scholar]
  17. MacLeod R. M., Lehmeyer J. E. Release of pituitary growth hormone by prostaglandins and dibutyryl adenosine cyclic 3':5'-monophosphate in the absence of protein synthesis. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1172–1179. doi: 10.1073/pnas.67.3.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pieri L., Keller H. H., Burkard W., Da Prada M. Effects of lisuride and LSD on cerebral monoamine systems and hallucinosis. Nature. 1978 Mar 16;272(5650):278–280. doi: 10.1038/272278a0. [DOI] [PubMed] [Google Scholar]
  19. Rodbell M. The role of hormone receptors and GTP-regulatory proteins in membrane transduction. Nature. 1980 Mar 6;284(5751):17–22. doi: 10.1038/284017a0. [DOI] [PubMed] [Google Scholar]
  20. Said S. I., Mutt V. Polypeptide with broad biological activity: isolation from small intestine. Science. 1970 Sep 18;169(3951):1217–1218. doi: 10.1126/science.169.3951.1217. [DOI] [PubMed] [Google Scholar]
  21. Salomon Y., Londos C., Rodbell M. A highly sensitive adenylate cyclase assay. Anal Biochem. 1974 Apr;58(2):541–548. doi: 10.1016/0003-2697(74)90222-x. [DOI] [PubMed] [Google Scholar]
  22. Schmidt M. J., Hill L. E. Effects of ergots on adenylate cyclase activity in the corpus striatum and pituitary. Life Sci. 1977 Mar 1;20(5):789–797. doi: 10.1016/0024-3205(77)90028-5. [DOI] [PubMed] [Google Scholar]
  23. Sibley D. R., Creese I. Guanine nucleotides regulate anterior pituitary dopamine receptors. Eur J Pharmacol. 1979 May 1;55(3):341–343. doi: 10.1016/0014-2999(79)90207-3. [DOI] [PubMed] [Google Scholar]
  24. Spano P. F., Govoni S., Trabucchi M. Studies on the pharmacological properties of dopamine receptors in various areas of the central nervous system. Adv Biochem Psychopharmacol. 1978;19:155–165. [PubMed] [Google Scholar]
  25. Sundberg D. K., Fawcett C. P., Illner P., McCann S. M. The effect of various prostaglandins and a prostaglandin synthetase inhibitor on rat anterior pituitary cyclic AMP levels and hormone release in vitro (38475). Proc Soc Exp Biol Med. 1975 Jan;148(1):54–59. doi: 10.3181/00379727-148-38475. [DOI] [PubMed] [Google Scholar]
  26. Theodorou A. E., Hall M. D., Jenner P., Marsden C. D. Cation regulation differentiates specific binding of [3H]sulpiride and [3H]spiperone to rat striatal preparations. J Pharm Pharmacol. 1980 Jun;32(6):441–444. doi: 10.1111/j.2042-7158.1980.tb12965.x. [DOI] [PubMed] [Google Scholar]
  27. Trabucchi M., Longoni R., Fresia P., Spano P. F. Sulpiride: a study of the effects on dopamine receptors in rat neostriatum and limbic forebrain. Life Sci. 1975 Nov 15;17(10):1551–1556. doi: 10.1016/0024-3205(75)90176-9. [DOI] [PubMed] [Google Scholar]
  28. Trabucchi M., Spano P. F., Tonon G. C., Frattola L. Effects of bromocriptine on central dopaminergic receptors. Life Sci. 1976 Jul 15;19(2):225–231. doi: 10.1016/0024-3205(76)90394-5. [DOI] [PubMed] [Google Scholar]
  29. Vijayan E., Samson W. K., Said S. I., McCann S. M. Vasoactive intestinal peptide: evidence for a hypothalamic site of action to release growth hormone, luteinizing hormone, and prolactin in conscious ovariectomized rats. Endocrinology. 1979 Jan;104(1):53–57. doi: 10.1210/endo-104-1-53. [DOI] [PubMed] [Google Scholar]
  30. Zor U., Kaneko T., Schneider H. P., McCann S. M., Lowe I. P., Bloom G., Borland B., Field J. B. Stimulation of anterior pituitary adenyl cyclase activity and adenosine 3':5'-cyclic phosphate by hypothalamic extract and prostaglandin E1. Proc Natl Acad Sci U S A. 1969 Jul;63(3):918–925. doi: 10.1073/pnas.63.3.918. [DOI] [PMC free article] [PubMed] [Google Scholar]

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