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. 1981 Nov;78(11):6826–6830. doi: 10.1073/pnas.78.11.6826

Cloning and sequence of cDNA coding for alpha 1-antitrypsin.

K Kurachi, T Chandra, S J Degen, T T White, T L Marchioro, S L Woo, E W Davie
PMCID: PMC349144  PMID: 7031661

Abstract

Recombinant plasmids containing human and baboon cDNA have been screened for alpha 1-antitrypsin, a major serine protease inhibitor present in blood. One plasmid, designated pBa alpha 1a2, was found to contain a cDNA insert of 1352 base pairs coding for the baboon inhibitor. It included 45 nucleotides that code for 15 amino acids present in the amino-terminal signal sequence of the protein, 1182 nucleotides that code for 394 amino acids in the mature protein, a stop codon, and a noncoding region of 76 nucleotides. Comparison of the amino acid sequences of baboon alpha 1-antitrypsin, human antithrombin III, and chicken ovalbumin indicated that these three proteins are about 230% homologous. A second plasmid, designated pH alpha 1a1, was found to contain a human cDNA insert of 306 base pairs. This plasmid coded for 69 amino acids present in the carboxyl-terminal region of human alpha 1-antitrypsin. The human and baboon cDNAs and their amino acid sequences are greater than 96% homologous.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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