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. 1981 Nov;78(11):7019–7023. doi: 10.1073/pnas.78.11.7019

RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.

N Hozumi, G E Wu, H Murialdo, L Roberts, D Vetter, W L Fife, M Whiteley, P Sadowski
PMCID: PMC349185  PMID: 6171827

Abstract

The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26, was found to contain a rearranged lambda I gene in addition to a rearranged lambda II gene. The rearranged lambda I gene was cloned into bacteriophage lambda DNA and its structure was studied. The lambda I gene was found to have arisen by an aberrant recombination event that resulted in a single base insertion at the site of V-J region joining. In addition, the gene contained numerous point mutations in the vicinity of the junction of the V and J regions. Two point mutations occurred in the donor splice sequence normally used for the removal of the intron between the J and C regions, suggesting that the RNA synthesized from the aberrantly rearranged lambda I gene would be unable to undergo proper RNA splicing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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