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. 1981 Nov;78(11):7033–7037. doi: 10.1073/pnas.78.11.7033

Chi activity during transduction-associated recombination.

N A Dower, F W Stahl
PMCID: PMC349188  PMID: 7031667

Abstract

Chi is a genetic element that stimulates phage lambda recombination by the Escherichia coli recBC pathway during lytic infection [Stahl, F. W. (1979) Annu. Rev. Genet. 13, 7--24]. Herein we show that chi in lambda prophage influences exchange distribution in P1 phage-mediated transduction and in conjugation. This demonstration encourages the view that chi may influence genetic exchange in E. coli in the total absence of lambda.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROWN A., ARBER W. TEMPERATURE-SENSITIVE MUTANTS OF COLIPHAGE LAMBDA. Virology. 1964 Oct;24:237–239. doi: 10.1016/0042-6822(64)90114-x. [DOI] [PubMed] [Google Scholar]
  3. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  4. Chattoraj D. K., Crasemann J. M., Dower N., Faulds D., Faulds P., Malone R. E., Stahl F. W., Stahl M. M. Chi. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1063–1066. doi: 10.1101/sqb.1979.043.01.116. [DOI] [PubMed] [Google Scholar]
  5. Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
  6. Faulds D., Dower N., Stahl M. M., Stahl F. W. Orientation-dependent recombination hotspot activity in bacteriophage lambda. J Mol Biol. 1979 Jul 15;131(4):681–695. doi: 10.1016/0022-2836(79)90197-9. [DOI] [PubMed] [Google Scholar]
  7. Feiss M., Adyha S., Court D. L. Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics. 1972 Jun;71(2):189–206. doi: 10.1093/genetics/71.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gussin G. N., Peterson V. Isolation and properties of rex - mutants of bacteriophage lambda. J Virol. 1972 Oct;10(4):760–765. doi: 10.1128/jvi.10.4.760-765.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Henderson D., Weil J. Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations. Genetics. 1975 Feb;79(2):143–174. doi: 10.1093/genetics/79.2.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JACOB F., WOLLMAN E. L. Etude génétique d'un bactériophage tempéré d'Escherichia coli. l. Le système genétique du bactériophage. Ann Inst Pasteur (Paris) 1954 Dec;87(6):653–673. [PubMed] [Google Scholar]
  11. KAISER A. D. The production of phage chromosome fragments and their capacity for genetic transfer. J Mol Biol. 1962 Apr;4:275–287. doi: 10.1016/s0022-2836(62)80005-9. [DOI] [PubMed] [Google Scholar]
  12. Kayajanian G. Deletion mapping of the c3-c2 region of bacteriophage lambda. Virology. 1970 May;41(1):170–174. doi: 10.1016/0042-6822(70)90065-6. [DOI] [PubMed] [Google Scholar]
  13. Lam S. T., Stahl M. M., McMilin K. D., Stahl F. W. Rec-mediated recombinational hot spot activity in bacteriophage lambda. II. A mutation which causes hot spot activity. Genetics. 1974 Jul;77(3):425–433. doi: 10.1093/genetics/77.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MESELSON M. ON THE MECHANISM OF GENETIC RECOMBINATION BETWEEN DNA MOLECULES. J Mol Biol. 1964 Sep;9:734–745. doi: 10.1016/s0022-2836(64)80178-9. [DOI] [PubMed] [Google Scholar]
  15. Malone R. E., Chattoraj D. K., Faulds D. H., Stahl M. M., Stahl F. W. Hotspots for generalized recombination in the Escherichia coli chromosome. J Mol Biol. 1978 Jun 5;121(4):473–491. doi: 10.1016/0022-2836(78)90395-9. [DOI] [PubMed] [Google Scholar]
  16. McMilin K. D., Stahl M. M., Stahl F. W. Rec-mediated recombinational hot spot activity in bacteriophage lambda. I. Hot spot activity associated with spi-deletions and bio substitutions. Genetics. 1974 Jul;77(3):409–423. doi: 10.1093/genetics/77.3.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rothman J. L. Transduction studies on the relation between prophage and host chromosome. J Mol Biol. 1965 Jul;12(3):892–912. doi: 10.1016/s0022-2836(65)80336-9. [DOI] [PubMed] [Google Scholar]
  18. Smith G. R., Comb M., Schultz D. W., Daniels D. L., Blattner F. R. Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda. J Virol. 1981 Jan;37(1):336–342. doi: 10.1128/jvi.37.1.336-342.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith G. R., Kunes S. M., Schultz D. W., Taylor A., Triman K. L. Structure of chi hotspots of generalized recombination. Cell. 1981 May;24(2):429–436. doi: 10.1016/0092-8674(81)90333-0. [DOI] [PubMed] [Google Scholar]
  20. Stahl F. W., Crasemann J. M., Stahl M. M. Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination. J Mol Biol. 1975 May 15;94(2):203–212. doi: 10.1016/0022-2836(75)90078-9. [DOI] [PubMed] [Google Scholar]
  21. Stahl F. W., McMilin K. D., Stahl M. M., Crasemann J. M., Lam S. The distribution of crossovers along unreplicated lambda bacteriophage chromosomes. Genetics. 1974 Jul;77(3):395–408. doi: 10.1093/genetics/77.3.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stahl F. W., McMilin K. D., Stahl M. M., Malone R. E., Nozu Y., Russo V. E. A role for recombination in the production of "free-loader" lambda bacteriophage particles. J Mol Biol. 1972 Jul 14;68(1):57–67. doi: 10.1016/0022-2836(72)90262-8. [DOI] [PubMed] [Google Scholar]
  23. Stahl F. W. Special sites in generalized recombination. Annu Rev Genet. 1979;13:7–24. doi: 10.1146/annurev.ge.13.120179.000255. [DOI] [PubMed] [Google Scholar]
  24. Stahl F. W., Stahl M. M., Malone R. E., Crasemann J. M. Directionality and nonreciprocality of Chi-stimulated recombination in phage lambda. Genetics. 1980 Feb;94(2):235–248. doi: 10.1093/genetics/94.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stahl F. W., Stahl M. M. Rec-mediated recombinational hot spot activity in bacteriophage lambda. IV. Effect of heterology on Chi-stimulated crossing over. Mol Gen Genet. 1975 Sep 15;140(1):29–37. doi: 10.1007/BF00268986. [DOI] [PubMed] [Google Scholar]
  26. Stahl F. W., Stahl M. M. Recombination pathway specificity of Chi. Genetics. 1977 Aug;86(4):715–725. doi: 10.1093/genetics/86.4.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas M., Cameron J. R., Davis R. W. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weigle J. Assembly of phage lambda in vitro. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1462–1466. doi: 10.1073/pnas.55.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]

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