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. 1981 Nov;78(11):7205–7209. doi: 10.1073/pnas.78.11.7205

Growth of purified astrocytes in a chemically defined medium.

R S Morrison, J de Vellis
PMCID: PMC349225  PMID: 6458820

Abstract

Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2 alpha, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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