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. 1981 Dec;78(12):7341–7345. doi: 10.1073/pnas.78.12.7341

Replication of linear mitochondrial DNA from Paramecium: sequence and structure of the initiation-end crosslink.

A E Pritchard, D J Cummings
PMCID: PMC349262  PMID: 6950379

Abstract

Replication of the 14-micrometer linear Paramecium mitochondrial DNA is initiated by a crosslinking of the duplex strands at the initiation end of the molecule. As a consequence of the crosslink, a head-to-head dimer molecule, or palindrome, is a replicative intermediate. The central region of the dimer molecules of two species was cloned and sequenced, In the distal regions, the sequence is palindromic as expected; however, in the central region, there is a nonpalindromic sequence that is rich in A + T and contains direct tandem repeats. It is proposed that the nonpalindromic sequence constitutes the crosslink. Implications for the replication scheme are discussed. The model is unlike any other for a linear DNA.

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Selected References

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