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. 1981 Dec;78(12):7346–7349. doi: 10.1073/pnas.78.12.7346

Spin trapping of free radicals during hepatic microsomal lipid peroxidation.

G M Rosen, E J Rauckman
PMCID: PMC349263  PMID: 6278469

Abstract

We have used spin-trapping techniques to identify radical species formed during the NADPH-stimulated peroxidation of rat hepatic microsomes. Using 5,5-dimethyl-1-pyrroline-1-oxide, we have confirmed the presence of substantial quantities of superoxide but found evidence for the formation of only small quantities of hydroxyl radical. Use of the spin traps N-tert-butyl-alpha-4-pyridylnitrone-1-oxide and 2-methyl-2-nitroso-1-propanol have allowed us to determine that lipid peroxyl radicals are the predominant "lipid-type" radical found in peroxidizing microsomes under aerobic conditions. These data suggest that microsomal lipid dienyl radicals react with molecular oxygen at near diffusion-controlled rates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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