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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1981 Dec;78(12):7727–7731. doi: 10.1073/pnas.78.12.7727

High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

B D Young, M A Ferguson-Smith, R Sillar, E Boyd
PMCID: PMC349343  PMID: 6950411

Abstract

A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of variation in the range 1-2%. At this level of resolution the contribution of many of the human chromosomes can be evaluated separately. Significant and reproducible differences between normal individuals have been detected and have been correlated with differences in the centric heterochromatin of certain chromosomes as revealed in their C-banded karyotypes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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