Abstract
Cultures of neurons from rat superior cervical ganglia were deprived of nerve growth factor, loaded with [methyl-3H]methionine, and then challenged with nerve growth factor for different periods of time. Growing neurites and perikarya were separated microsurgically and extracted with chloroform/methanol. Lipid-incorporated radioactivity in the extracts was measured and expressed on the basis of the amount of phospholipid present. The methylated species in the neurite fraction were identified by thin-layer chromatography as mono-, di-, and trimethylphosphatidylethanolamine (phosphatidylcholine). Furthermore, a small peak of lysophosphatidylcholine was detected. In the neurites, but not in the perikarya, phospholipid methylation was found to reach a peak at 10 sec after onset of stimulation. Stimulated levels were at least 4 times higher than levels of unstimulated controls. The peak was followed by rapid decline of phospholipid-incorporated radioactivity. Our result indicates that phospholipid methylation is part of a nerve-growth-factor-activated secondary messenger system in growing sympathetic neurites. The potential significance of this conclusion for directed neuritic growth and membrane expansion is discussed.
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Selected References
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- AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
- Banerjee S. P., Snyder S. H., Cuatrecasas P., Greene L. A. Binding of nerve growth factor receptor in sympathetic ganglia. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2519–2523. doi: 10.1073/pnas.70.9.2519. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bray D. Branching patterns of individual sympathetic neurons in culture. J Cell Biol. 1973 Mar;56(3):702–712. doi: 10.1083/jcb.56.3.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bray D. Surface movements during the growth of single explanted neurons. Proc Natl Acad Sci U S A. 1970 Apr;65(4):905–910. doi: 10.1073/pnas.65.4.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campenot R. B. Local control of neurite development by nerve growth factor. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4516–4519. doi: 10.1073/pnas.74.10.4516. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frazier W. A., Boyd L. F., Bradshaw R. A. Properties of the specific binding of 125I-nerve growth factor to responsive peripheral neurons. J Biol Chem. 1974 Sep 10;249(17):5513–5519. [PubMed] [Google Scholar]
- Greene L. A., Shooter E. M. The nerve growth factor: biochemistry, synthesis, and mechanism of action. Annu Rev Neurosci. 1980;3:353–402. doi: 10.1146/annurev.ne.03.030180.002033. [DOI] [PubMed] [Google Scholar]
- Gundersen R. W., Barrett J. N. Characterization of the turning response of dorsal root neurites toward nerve growth factor. J Cell Biol. 1980 Dec;87(3 Pt 1):546–554. doi: 10.1083/jcb.87.3.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatanaka H., Otten U., Thoenen H. Nerve growth factor-mediated selective induction of ornithine decarboxylase in rat pheochromocytoma; a cyclic AMP-independent process. FEBS Lett. 1978 Aug 15;92(2):313–316. doi: 10.1016/0014-5793(78)80777-7. [DOI] [PubMed] [Google Scholar]
- Herrup K., Shooter E. M. Properties of the beta nerve growth factor receptor of avian dorsal root ganglia. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3884–3888. doi: 10.1073/pnas.70.12.3884. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heumann R., Schwab M., Thoenen H. A second messenger required for nerve growth factor biological activity? Nature. 1981 Aug 27;292(5826):838–840. doi: 10.1038/292838a0. [DOI] [PubMed] [Google Scholar]
- Hier D. B., Arnason B. G., Young M. Studies on the mechanism of action of nerve growth factor. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2268–2272. doi: 10.1073/pnas.69.8.2268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirata F., Axelrod J. Phospholipid methylation and biological signal transmission. Science. 1980 Sep 5;209(4461):1082–1090. doi: 10.1126/science.6157192. [DOI] [PubMed] [Google Scholar]
- Hirata F., Corcoran B. A., Venkatasubramanian K., Schiffmann E., Axelrod J. Chemoattractants stimulate degradation of methylated phospholipids and release of arachidonic acid in rabbit leukocytes. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2640–2643. doi: 10.1073/pnas.76.6.2640. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirata F., Viveros O. H., Diliberto E. J., Jr, Axelrod J. Identification and properties of two methyltransferases in conversion of phosphatidylethanolamine to phosphatidylcholine. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1718–1721. doi: 10.1073/pnas.75.4.1718. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishizaka T., Hirata F., Ishizaka K., Axelrod J. Stimulation of phospholipid methylation, Ca2+ influx, and histamine release by bridging of IgE receptors on rat mast cells. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1903–1906. doi: 10.1073/pnas.77.4.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landreth G., Cohen P., Shooter E. M. Ca2+ transmembrane fluxes and nerve growth factor action on a clonal cell line of rat phaeochromocytoma. Nature. 1980 Jan 10;283(5743):202–204. doi: 10.1038/283202a0. [DOI] [PubMed] [Google Scholar]
- Letourneau P. C. Chemotactic response of nerve fiber elongation to nerve growth factor. Dev Biol. 1978 Sep;66(1):183–196. doi: 10.1016/0012-1606(78)90283-x. [DOI] [PubMed] [Google Scholar]
- Meiri H., Spira M. E., Parnas I. Membrane conductance and action potential of a regenerating axonal tip. Science. 1981 Feb 13;211(4483):709–712. doi: 10.1126/science.7455707. [DOI] [PubMed] [Google Scholar]
- Pfenninger K. H., Maylié-Pfenninger M. F. Lectin labeling of sprouting neurons. II. Relative movement and appearance of glycoconjugates during plasmalemmal expansion. J Cell Biol. 1981 Jun;89(3):547–559. doi: 10.1083/jcb.89.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Poole A. R., Howell J. I., Lucy J. A. Lysolecithin and cell fusion. Nature. 1970 Aug 22;227(5260):810–814. doi: 10.1038/227810a0. [DOI] [PubMed] [Google Scholar]
- Schubert D., LaCorbiere M., Whitlock C., Stallcup W. Alterations in the surface properties of cells responsive to nerve growth factor. Nature. 1978 Jun 29;273(5665):718–723. doi: 10.1038/273718a0. [DOI] [PubMed] [Google Scholar]
- Schubert D., Whitlock C. Alteration of cellular adhesion by nerve growth factor. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4055–4058. doi: 10.1073/pnas.74.9.4055. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherbany A. A., Ambron R. T., Schwartz J. H. Membrane glycolipids: regional synthesis and axonal transport in a single identified neuron of Aplysia californica. Science. 1979 Jan 5;203(4375):78–80. doi: 10.1126/science.83001. [DOI] [PubMed] [Google Scholar]
- Stöckel K., Paravicini U., Thoenen H. Specificity of the retrograde axonal transport of nerve growth factor. Brain Res. 1974 Aug 23;76(3):413–421. doi: 10.1016/0006-8993(74)90818-x. [DOI] [PubMed] [Google Scholar]
- Suda K., Barde Y. A., Thoenen H. Nerve growth factor in mouse and rat serum: correlation between bioassay and radioimmunoassay determinations. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4042–4046. doi: 10.1073/pnas.75.8.4042. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yu M. W., Tolson N. W., Guroff G. Increased phosphorylation of specific nuclear proteins in superior cervical ganglia and PC12 cells in response to nerve growth factor. J Biol Chem. 1980 Nov 10;255(21):10481–10492. [PubMed] [Google Scholar]