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. 1981 Dec;78(12):7825–7829. doi: 10.1073/pnas.78.12.7825

Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.

R Van Buskirk, J E Dowling
PMCID: PMC349364  PMID: 6278491

Abstract

Horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. Fractions containing horizontal cells were tested for their ability to accumulate cyclic AMP in the presence of various putative neurotransmitters. Micromolar concentrations of dopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic AMP accumulation in these isolated cells. The dopamine-dependent accumulation of cyclic AMP in intact isolated horizontal cells was blocked by nanomolar concentrations of dopamine antagonists such as haloperidol, (+)-butaclamol, and fluphenazine. The results indicate that there is a postsynaptic dopamine receptor on carp horizontal cells that is associated with adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1].

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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