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. 1980 May;77(5):2474–2476. doi: 10.1073/pnas.77.5.2474

Measurements of the pH within dormant and germinated bacterial spores.

B Setlow, P Setlow
PMCID: PMC349422  PMID: 6771755

Abstract

The pH within the core or central region of dormant spores of Bacillus cereus and B. megaterium is 6.3-6.4 irrespective of the external pH. However, the spore's internal pH rises to 7.3-7.5 upon germination. The low internal pH of the dormant spore may be a contributing factor to its metabolic dormancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACK S. H., GERHARDT P. Permeability of bacterial spores. IV. Water content, uptake, and distribution. J Bacteriol. 1962 May;83:960–967. doi: 10.1128/jb.83.5.960-967.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barton J. K., den Hollander J. A., Lee T. M., MacLaughlin A., Shulman R. G. Measurement of the internal pH of yeast spores by 31P nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1980 May;77(5):2470–2473. doi: 10.1073/pnas.77.5.2470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHURCH B. D., HALVORSON H. Intermediate metabolism of aerobic spores. I. Activation of glucose oxidation in spores of Bacillus cereus var terminalis. J Bacteriol. 1957 Apr;73(4):470–476. doi: 10.1128/jb.73.4.470-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carstensen E. L., Marquis R. E., Gerhardt P. Dielectric study of the physical state of electrolytes and water within Bacillus cereus spores. J Bacteriol. 1971 Jul;107(1):106–113. doi: 10.1128/jb.107.1.106-113.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GERHARDT P., BLACK S. H. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J Bacteriol. 1961 Nov;82:750–760. doi: 10.1128/jb.82.5.750-760.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson J. D., Epel D. Intracellular pH and activation of sea urchin eggs after fertilisation. Nature. 1976 Aug 19;262(5570):661–664. doi: 10.1038/262661a0. [DOI] [PubMed] [Google Scholar]
  7. Johnson R. G., Scarpa A. Ion permeability of isolated chromaffin granules. J Gen Physiol. 1976 Dec;68(6):601–631. doi: 10.1085/jgp.68.6.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kashket E. R., Wilson T. H. Proton-coupled accumulation of galactoside in Streptococcus lactis 7962. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2866–2869. doi: 10.1073/pnas.70.10.2866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sadoff H. L. Symposium on bacterial spores: X. Heat resistance of spore enzymes. J Appl Bacteriol. 1970 Mar;33(1):130–140. doi: 10.1111/j.1365-2672.1970.tb05238.x. [DOI] [PubMed] [Google Scholar]
  10. Setlow P. Identification and localization of the major proteins degraded during germination of Bacillus megaterium spores. J Biol Chem. 1975 Oct 25;250(20):8159–8167. [PubMed] [Google Scholar]
  11. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination. J Biol Chem. 1970 Jul 25;245(14):3645–3652. [PubMed] [Google Scholar]
  12. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination. J Bacteriol. 1969 Dec;100(3):1155–1160. doi: 10.1128/jb.100.3.1155-1160.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores. J Biol Chem. 1970 Jul 25;245(14):3637–3644. [PubMed] [Google Scholar]
  14. Singh R. P., Setlow B., Setlow P. Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium. J Bacteriol. 1977 Jun;130(3):1130–1138. doi: 10.1128/jb.130.3.1130-1138.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Singh R. P., Setlow P. Phosphoglycerate mutase in developing forespores of Bacillus megaterium may be regulated by the intrasporal level of free manganous ion. Biochem Biophys Res Commun. 1978 May 15;82(1):1–5. doi: 10.1016/0006-291x(78)90567-3. [DOI] [PubMed] [Google Scholar]
  16. Singh R. P., Setlow P. Purification and properties of phosphoglycerate phosphomutase from spores and cells of Bacillus megaterium. J Bacteriol. 1979 Feb;137(2):1024–1027. doi: 10.1128/jb.137.2.1024-1027.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wyatt P. J. Observations on the structure of spores. J Appl Bacteriol. 1975 Feb;38(1):47–51. doi: 10.1111/j.1365-2672.1975.tb00499.x. [DOI] [PubMed] [Google Scholar]

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