Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 May;77(5):2514–2518. doi: 10.1073/pnas.77.5.2514

Fate of donor insertion sequence IS1 during transposition.

H A Read, S Das Sarma, S R Jaskunas
PMCID: PMC349431  PMID: 6248852

Abstract

The number of insertion sequence IS1 segments in Escherichia coli K-12 and 20 mutants in which an ISI had been inserted at a new site was measured by Southern blot hybridization analysis. The parent strain appeared to contain seven IS1 segments. Each of the mutants contained these seven IS1 and one additional IS1 corresponding to the new IS1 insertion. These results suggest that a copy of a donor ISI is inserted at the new site. A model for transposition is prevented that postulates that a reactive intermediate is formed by a tandem duplication of a transposable sequence.

Full text

PDF
2514

Images in this article

2515Image
on p.2515
2516Image
on p.2516
2516Image
on p.2516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett P. M., Grinsted J., Richmond M. H. Transposition of TnA does not generate deletions. Mol Gen Genet. 1977 Jul 20;154(2):205–211. doi: 10.1007/BF00330839. [DOI] [PubMed] [Google Scholar]
  2. Calos M. P., Johnsrud L., Miller J. H. DNA sequence at the integration sites of the insertion element IS1. Cell. 1978 Mar;13(3):411–418. doi: 10.1016/0092-8674(78)90315-x. [DOI] [PubMed] [Google Scholar]
  3. Cameron J. R., Loh E. Y., Davis R. W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 1979 Apr;16(4):739–751. doi: 10.1016/0092-8674(79)90090-4. [DOI] [PubMed] [Google Scholar]
  4. Eisenberg S., Griffith J., Kornberg A. phiX174 cistron A protein is a multifunctional enzyme in DNA replication. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3198–3202. doi: 10.1073/pnas.74.8.3198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Faelen M., Toussaint A., De Lafonteyne J. Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens. J Bacteriol. 1975 Mar;121(3):873–882. doi: 10.1128/jb.121.3.873-882.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fiandt M., Szybalski W., Blattner F. R., Jaskunas S. R., Lindahl L., Nomura M. Organization of ribosomal protein genes in Escherichia coli. I. Physical structure of DNA from transducing lambda phages carrying genes from the aroE-str region. J Mol Biol. 1976 Sep 25;106(3):817–835. doi: 10.1016/0022-2836(76)90267-9. [DOI] [PubMed] [Google Scholar]
  7. Fincham J. R., Sastry G. R. Controlling elements in maize. Annu Rev Genet. 1974;8:15–50. doi: 10.1146/annurev.ge.08.120174.000311. [DOI] [PubMed] [Google Scholar]
  8. Ghosal D., Saedler H. DNA sequence of the mini-insertion IS2--6 and its relation to the sequence of IS2. Nature. 1978 Oct 19;275(5681):611–617. doi: 10.1038/275611a0. [DOI] [PubMed] [Google Scholar]
  9. Gill R., Heffron F., Dougan G., Falkow S. Analysis of sequences transposed by complementation of two classes of transposition-deficient mutants of Tn3. J Bacteriol. 1978 Nov;136(2):742–756. doi: 10.1128/jb.136.2.742-756.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gottesman M. M., Rosner J. L. Acquisition of a determinant for chloramphenicol resistance by coliphage lambda. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5041–5045. doi: 10.1073/pnas.72.12.5041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grindley N. D. IS1 insertion generates duplication of a nine base pair sequence at its target site. Cell. 1978 Mar;13(3):419–426. doi: 10.1016/0092-8674(78)90316-1. [DOI] [PubMed] [Google Scholar]
  12. Grindley N. D., Sherratt D. J. Sequence analysis at IS1 insertion sites: models for transposition. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1257–1261. doi: 10.1101/sqb.1979.043.01.142. [DOI] [PubMed] [Google Scholar]
  13. Hughes S. H., Shank P. R., Spector D. H., Kung H. J., Bishop J. M., Varmus H. E., Vogt P. K., Breitman M. L. Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites. Cell. 1978 Dec;15(4):1397–1410. doi: 10.1016/0092-8674(78)90064-8. [DOI] [PubMed] [Google Scholar]
  14. Jaskunas S. R., Lindahl L., Nomura M. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli. Nature. 1975 Jul 17;256(5514):183–187. doi: 10.1038/256183a0. [DOI] [PubMed] [Google Scholar]
  15. Jaskunas S. R., Nomura M. Organization of ribosomal protein genes of Escherichia coli as analyzed by polar insertion mutations. J Biol Chem. 1977 Oct 25;252(20):7337–7343. [PubMed] [Google Scholar]
  16. Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
  17. Johnsrud L. DNA sequence of the transposable element IS1. Mol Gen Genet. 1979 Jan 31;169(2):213–218. doi: 10.1007/BF00271673. [DOI] [PubMed] [Google Scholar]
  18. Kleckner N. Translocatable elements in procaryotes. Cell. 1977 May;11(1):11–23. doi: 10.1016/0092-8674(77)90313-0. [DOI] [PubMed] [Google Scholar]
  19. Kühn S., Fritz H. J., Starlinger P. Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli. Mol Gen Genet. 1979 Jan 2;167(3):235–241. doi: 10.1007/BF00267414. [DOI] [PubMed] [Google Scholar]
  20. Ljungquist E., Bukhari A. I. State of prophage Mu DNA upon induction. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3143–3147. doi: 10.1073/pnas.74.8.3143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ohtsubo H., Ohtsubo E. Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):615–619. doi: 10.1073/pnas.75.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Potter S. S., Brorein W. J., Jr, Dunsmuir P., Rubin G. M. Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):415–427. doi: 10.1016/0092-8674(79)90168-5. [DOI] [PubMed] [Google Scholar]
  23. Ross D. G., Swan J., Kleckner N. Nearly precise excision: a new type of DNA alteration associated with the translocatable element Tn10. Cell. 1979 Apr;16(4):733–738. doi: 10.1016/0092-8674(79)90089-8. [DOI] [PubMed] [Google Scholar]
  24. Ross D. G., Swan J., Kleckner N. Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetitions. Cell. 1979 Apr;16(4):721–731. doi: 10.1016/0092-8674(79)90088-6. [DOI] [PubMed] [Google Scholar]
  25. Saedler H., Heiss B. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12. Mol Gen Genet. 1973 May 9;122(3):267–277. doi: 10.1007/BF00278602. [DOI] [PubMed] [Google Scholar]
  26. Saedler H., Kubai D. F., Nomura M., Jaskunas S. R. IS1 and IS2 mutations in the ribosomal protein genes of E. coli K-12. Mol Gen Genet. 1975 Nov 3;141(1):85–89. doi: 10.1007/BF00332381. [DOI] [PubMed] [Google Scholar]
  27. Shank P. R., Hughes S. H., Kung H. J., Majors J. E., Quintrell N., Guntaka R. V., Bishop J. M., Varmus H. E. Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. Cell. 1978 Dec;15(4):1383–1395. doi: 10.1016/0092-8674(78)90063-6. [DOI] [PubMed] [Google Scholar]
  28. Shapiro J. A. Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1933–1937. doi: 10.1073/pnas.76.4.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shinnick T. M., Lund E., Smithies O., Blattner F. R. Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res. 1975 Oct;2(10):1911–1929. doi: 10.1093/nar/2.10.1911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  31. Starlinger P. DNA rearrangements in procaryotes. Annu Rev Genet. 1977;11:103–126. doi: 10.1146/annurev.ge.11.120177.000535. [DOI] [PubMed] [Google Scholar]
  32. Strobel E., Dunsmuir P., Rubin G. M. Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979 Jun;17(2):429–439. doi: 10.1016/0092-8674(79)90169-7. [DOI] [PubMed] [Google Scholar]
  33. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES